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| | <StructureSection load='2ww5' size='340' side='right'caption='[[2ww5]], [[Resolution|resolution]] 1.61Å' scene=''> | | <StructureSection load='2ww5' size='340' side='right'caption='[[2ww5]], [[Resolution|resolution]] 1.61Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2ww5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strr6 Strr6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WW5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2WW5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2ww5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_R6 Streptococcus pneumoniae R6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WW5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WW5 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CHT:CHOLINE+ION'>CHT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.61Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wwd|2wwd]], [[2wwc|2wwc]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CHT:CHOLINE+ION'>CHT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ww5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ww5 OCA], [https://pdbe.org/2ww5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ww5 RCSB], [https://www.ebi.ac.uk/pdbsum/2ww5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ww5 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2ww5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ww5 OCA], [http://pdbe.org/2ww5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ww5 RCSB], [http://www.ebi.ac.uk/pdbsum/2ww5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ww5 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8DP07_STRR6 Q8DP07_STRR6] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lysozyme]] | + | [[Category: Streptococcus pneumoniae R6]] |
| - | [[Category: Strr6]]
| + | [[Category: Garcia A]] |
| - | [[Category: Garcia, A]] | + | [[Category: Garcia JL]] |
| - | [[Category: Garcia, J L]] | + | [[Category: Garcia P]] |
| - | [[Category: Garcia, P]] | + | [[Category: Gonzalez A]] |
| - | [[Category: Gonzalez, A]] | + | [[Category: Hermoso JA]] |
| - | [[Category: Hermoso, J A]] | + | [[Category: Menendez M]] |
| - | [[Category: Menendez, M]] | + | [[Category: Perez-Dorado I]] |
| - | [[Category: Perez-Dorado, I]] | + | [[Category: Sanles R]] |
| - | [[Category: Sanles, R]] | + | |
| - | [[Category: Choline-binding protein]]
| + | |
| - | [[Category: Glycosidase]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
Q8DP07_STRR6
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The first structure of a pneumococcal autolysin, that of the LytC lysozyme, has been solved in ternary complex with choline and a pneumococcal peptidoglycan (PG) fragment. The active site of the hydrolase module is not fully exposed but is oriented toward the choline-binding module, which accounts for its unique in vivo features in PG hydrolysis, its activation and its regulatory mechanisms. Because of the unusual hook-shaped conformation of the multimodular protein, it is only able to hydrolyze non-cross-linked PG chains, an assertion validated by additional experiments. These results explain the activation of LytC by choline-binding protein D (CbpD) in fratricide, a competence-programmed mechanism of predation of noncompetent sister cells. The results provide the first structural insights to our knowledge into the critical and central function that LytC plays in pneumococcal virulence and explain a long-standing puzzle of how murein hydrolases can be controlled to avoid self-lysis during bacterial growth and division.
Insights into pneumococcal fratricide from the crystal structures of the modular killing factor LytC.,Perez-Dorado I, Gonzalez A, Morales M, Sanles R, Striker W, Vollmer W, Mobashery S, Garcia JL, Martinez-Ripoll M, Garcia P, Hermoso JA Nat Struct Mol Biol. 2010 May;17(5):576-81. Epub 2010 Apr 18. PMID:20400948[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Perez-Dorado I, Gonzalez A, Morales M, Sanles R, Striker W, Vollmer W, Mobashery S, Garcia JL, Martinez-Ripoll M, Garcia P, Hermoso JA. Insights into pneumococcal fratricide from the crystal structures of the modular killing factor LytC. Nat Struct Mol Biol. 2010 May;17(5):576-81. Epub 2010 Apr 18. PMID:20400948 doi:10.1038/nsmb.1817
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