6yai
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Clathrin with bound beta2 appendage of AP2== | |
| + | <StructureSection load='6yai' size='340' side='right'caption='[[6yai]], [[Resolution|resolution]] 9.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6yai]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YAI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YAI FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 9.2Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yai OCA], [https://pdbe.org/6yai PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yai RCSB], [https://www.ebi.ac.uk/pdbsum/6yai PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yai ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/I3LGD4_PIG I3LGD4_PIG] Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.[PIRNR:PIRNR002290] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Clathrin-mediated endocytosis (CME) is crucial for modulating the protein composition of a cell's plasma membrane. Clathrin forms a cage-like, polyhedral outer scaffold around a vesicle, to which cargo-selecting clathrin adaptors are attached. Adaptor protein complex (AP2) is the key adaptor in CME. Crystallography has shown AP2 to adopt a range of conformations. Here, we used cryo-electron microscopy, tomography, and subtomogram averaging to determine structures, interactions, and arrangements of clathrin and AP2 at the key steps of coat assembly, from AP2 in solution to membrane-assembled clathrin-coated vesicles (CCVs). AP2 binds cargo and PtdIns(4,5)P 2 (phosphatidylinositol 4,5-bisphosphate)-containing membranes via multiple interfaces, undergoing conformational rearrangement from its cytosolic state. The binding mode of AP2 beta2 appendage into the clathrin lattice in CCVs and buds implies how the adaptor structurally modulates coat curvature and coat disassembly. | ||
| - | + | Architecture of the AP2/clathrin coat on the membranes of clathrin-coated vesicles.,Kovtun O, Dickson VK, Kelly BT, Owen DJ, Briggs JAG Sci Adv. 2020 Jul 22;6(30):eaba8381. doi: 10.1126/sciadv.aba8381. eCollection, 2020 Jul. PMID:32743075<ref>PMID:32743075</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Briggs | + | <div class="pdbe-citations 6yai" style="background-color:#fffaf0;"></div> |
| - | [[Category: Kane Dickson | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[Adaptin 3D structures|Adaptin 3D structures]] |
| - | [[Category: Owen | + | *[[Clathrin 3D structures|Clathrin 3D structures]] |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Sus scrofa]] | ||
| + | [[Category: Briggs JAG]] | ||
| + | [[Category: Kane Dickson V]] | ||
| + | [[Category: Kelly BT]] | ||
| + | [[Category: Kovtun O]] | ||
| + | [[Category: Owen D]] | ||
Current revision
Clathrin with bound beta2 appendage of AP2
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