This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
7chd
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 7chd is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures) |
|||
| (2 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==AtaT complexed with acetyl-methionyl-tRNAfMet== | |
| + | <StructureSection load='7chd' size='340' side='right'caption='[[7chd]], [[Resolution|resolution]] 3.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7chd]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CHD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CHD FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.804Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7chd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7chd OCA], [https://pdbe.org/7chd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7chd RCSB], [https://www.ebi.ac.uk/pdbsum/7chd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7chd ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q8XED1_ECO57 Q8XED1_ECO57] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Toxin-antitoxin systems in bacteria contribute to stress adaptation, dormancy, and persistence. AtaT, a type-II toxin in enterohemorrhagic E. coli, reportedly acetylates the alpha-amino group of the aminoacyl-moiety of initiator Met-tRNAf(Met), thus inhibiting translation initiation. Here, we show that AtaT has a broader specificity for aminoacyl-tRNAs than initially claimed. AtaT efficiently acetylates Gly-tRNA(Gly), Trp-tRNA(Trp), Tyr-tRNA(Tyr) and Phe-tRNA(Phe) isoacceptors, in addition to Met-tRNAf(Met), and inhibits global translation. AtaT interacts with the acceptor stem of tRNAf(Met), and the consecutive G-C pairs in the bottom-half of the acceptor stem are required for acetylation. Consistently, tRNA(Gly), tRNA(Trp), tRNA(Tyr) and tRNA(Phe) also possess consecutive G-C base-pairs in the bottom halves of their acceptor stems. Furthermore, misaminoacylated valyl-tRNAf(Met) and isoleucyl-tRNAf(Met) are not acetylated by AtaT. Therefore, the substrate selection by AtaT is governed by the specific acceptor stem sequence and the properties of the aminoacyl-moiety of aminoacyl-tRNAs. | ||
| - | + | Mechanism of aminoacyl-tRNA acetylation by an aminoacyl-tRNA acetyltransferase AtaT from enterohemorrhagic E. coli.,Yashiro Y, Sakaguchi Y, Suzuki T, Tomita K Nat Commun. 2020 Oct 28;11(1):5438. doi: 10.1038/s41467-020-19281-z. PMID:33116145<ref>PMID:33116145</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7chd" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Escherichia coli]] | ||
| + | [[Category: Escherichia coli O157:H7]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Tomita K]] | ||
| + | [[Category: Yashiro Y]] | ||
Current revision
AtaT complexed with acetyl-methionyl-tRNAfMet
| |||||||||||
