6z6s

==Crystal structure of Uba4-Urm1 from Chaetomium thermophilum==

<StructureSection load='6z6s' size='340' side='right'caption='[[6z6s]], [[Resolution|resolution]] 3.15&Aring;' scene=''>

<table><tr><td colspan='2'>[[6z6s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z6S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z6S FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.153&#8491;</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z6s OCA], [https://pdbe.org/6z6s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z6s RCSB], [https://www.ebi.ac.uk/pdbsum/6z6s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z6s ProSAT]</span></td></tr>

== Function ==

[https://www.uniprot.org/uniprot/G0SC54_CHATD G0SC54_CHATD] Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers urm1 and MOCS2A. Its N-terminus first activates urm1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to urm1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards urm1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; nfs1 probably acting as a sulfur donor for thiocarboxylation reactions.[HAMAP-Rule:MF_03049]

[[Category: Chaetomium thermophilum var. thermophilum DSM 1495]]

[[Category: Ethiraju Ravichandran K]]

[[Category: Pabis M]]