6o6e

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<StructureSection load='6o6e' size='340' side='right'caption='[[6o6e]], [[Resolution|resolution]] 2.14&Aring;' scene=''>
<StructureSection load='6o6e' size='340' side='right'caption='[[6o6e]], [[Resolution|resolution]] 2.14&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6o6e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Psef5 Psef5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O6E OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6O6E FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6o6e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_protegens_Pf-5 Pseudomonas protegens Pf-5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O6E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6O6E FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=LOY:5-deoxy-5-[({(2R)-2-({2-[(N-{(2R)-4-[(dioxo-lambda~5~-phosphanyl)oxy]-2-hydroxy-3,3-dimethylbutanoyl}-beta-alanyl)amino]ethyl}sulfanyl)-2-[(2R)-pyrrolidin-2-yl]ethyl}sulfonyl)amino]adenosine'>LOY</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.14&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pltF, PFL_2792 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=220664 PSEF5]), pltL, PFL_2786 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=220664 PSEF5])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=I5M:(2~{R})-~{N}-[3-[2-[(1~{S})-2-[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methylsulfamoyl]-1-[(2~{S})-pyrrolidin-2-yl]ethyl]sulfanylethylamino]-3-oxidanylidene-propyl]-3,3-dimethyl-2-oxidanyl-4-phosphooxy-butanamide'>I5M</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-proline--[L-prolyl-carrier_protein]_ligase L-proline--[L-prolyl-carrier protein] ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.2.1.53 6.2.1.53] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6o6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o6e OCA], [https://pdbe.org/6o6e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6o6e RCSB], [https://www.ebi.ac.uk/pdbsum/6o6e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6o6e ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6o6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o6e OCA], [http://pdbe.org/6o6e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o6e RCSB], [http://www.ebi.ac.uk/pdbsum/6o6e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o6e ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/PLTF_PSEF5 PLTF_PSEF5]] Involved in the biosynthesis of pyoluteorin. Catalyzes the conversion of L-proline to L-prolyl-AMP and the transfer of the L-prolyl group to acyl carrier protein PltL.<ref>PMID:11880032</ref>
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[https://www.uniprot.org/uniprot/PLTF_PSEF5 PLTF_PSEF5] Involved in the biosynthesis of pyoluteorin. Catalyzes the conversion of L-proline to L-prolyl-AMP and the transfer of the L-prolyl group to acyl carrier protein PltL.<ref>PMID:11880032</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Using a covalent chemical probe and X-ray crystallography coupled to nuclear magnetic resonance data, we elucidated the dynamic molecular basis of protein recognition between the carrier protein and adenylation domain in pyoluteorin biosynthesis. These findings reveal a unique binding mode, which contrasts previously solved carrier protein and partner protein interfaces.
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Dynamic visualization of type II peptidyl carrier protein recognition in pyoluteorin biosynthesis.,Corpuz JC, Podust LM, Davis TD, Jaremko MJ, Burkart MD RSC chem biol. 2020 Apr 1;1(1):8-12. doi: 10.1039/c9cb00015a. Epub 2020 Mar 24. PMID:33305272<ref>PMID:33305272</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6o6e" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Psef5]]
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[[Category: Pseudomonas protegens Pf-5]]
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[[Category: Corpuz, J C]]
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[[Category: Corpuz JC]]
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[[Category: Podust, L M]]
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[[Category: Podust LM]]
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[[Category: Biosynthesis]]
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[[Category: Ligase-transport protein complex]]
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[[Category: Nonribosomal peptide synthetase]]
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[[Category: Nrp]]
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[[Category: Pyoluteorin]]
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[[Category: Type ii]]
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Current revision

Crystal structure of PltF trapped with PltL using a proline adenosine vinylsulfonamide inhibitor

PDB ID 6o6e

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