6x0o

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Current revision (14:47, 6 March 2024) (edit) (undo)
 
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<StructureSection load='6x0o' size='340' side='right'caption='[[6x0o]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
<StructureSection load='6x0o' size='340' side='right'caption='[[6x0o]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6x0o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X0O OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6X0O FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6x0o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X0O FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=LHG:1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE'>LHG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">embB, MSMEI_6221 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246196 MYCS2])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=LHG:1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE'>LHG</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Indolylacetylinositol_arabinosyltransferase Indolylacetylinositol arabinosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.34 2.4.2.34] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x0o OCA], [https://pdbe.org/6x0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x0o RCSB], [https://www.ebi.ac.uk/pdbsum/6x0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x0o ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6x0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x0o OCA], [http://pdbe.org/6x0o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6x0o RCSB], [http://www.ebi.ac.uk/pdbsum/6x0o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6x0o ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0R614_MYCS2 A0R614_MYCS2] Arabinosyl transferase responsible for the polymerization of arabinose into the arabinan of arabinogalactan.[ARBA:ARBA00003001]
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Arabinosyltransferase B (EmbB) belongs to a family of membrane-bound glycosyltransferases that build the lipidated polysaccharides of the mycobacterial cell envelope, and are targets of anti-tuberculosis drug ethambutol. We present the 3.3 A resolution single-particle cryo-electron microscopy structure of Mycobacterium smegmatis EmbB, providing insights on substrate binding and reaction mechanism. Mutations that confer ethambutol resistance map mostly around the putative active site, suggesting this to be the location of drug binding.
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Cryo-EM structure of arabinosyltransferase EmbB from Mycobacterium smegmatis.,Tan YZ, Rodrigues J, Keener JE, Zheng RB, Brunton R, Kloss B, Giacometti SI, Rosario AL, Zhang L, Niederweis M, Clarke OB, Lowary TL, Marty MT, Archer M, Potter CS, Carragher B, Mancia F Nat Commun. 2020 Jul 7;11(1):3396. doi: 10.1038/s41467-020-17202-8. PMID:32636380<ref>PMID:32636380</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6x0o" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Indolylacetylinositol arabinosyltransferase]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Mycs2]]
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[[Category: Mycolicibacterium smegmatis MC2 155]]
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[[Category: Archer, M]]
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[[Category: Archer M]]
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[[Category: Brunton, R]]
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[[Category: Brunton R]]
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[[Category: Carragher, B]]
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[[Category: Carragher B]]
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[[Category: Clarke, O B]]
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[[Category: Clarke OB]]
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[[Category: Giacometti, S I]]
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[[Category: Giacometti SI]]
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[[Category: Keener, J E]]
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[[Category: Keener JE]]
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[[Category: Kloss, B]]
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[[Category: Kloss B]]
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[[Category: Lowary, T L]]
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[[Category: Lowary TL]]
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[[Category: Mancia, F]]
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[[Category: Mancia F]]
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[[Category: Marty, M T]]
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[[Category: Marty MT]]
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[[Category: Niederweis, M]]
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[[Category: Niederweis M]]
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[[Category: Potter, C S]]
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[[Category: Potter CS]]
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[[Category: Rodrigues, J]]
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[[Category: Rodrigues J]]
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[[Category: Rosario, A L]]
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[[Category: Rosario AL]]
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[[Category: Tan, Y Z]]
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[[Category: Tan YZ]]
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[[Category: Zhang, L]]
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[[Category: Zhang L]]
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[[Category: Zheng, R B]]
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[[Category: Zheng RB]]
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[[Category: Glycosyltransferase]]
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[[Category: Membrane protein]]
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[[Category: Nanodisc]]
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Current revision

Single-Particle Cryo-EM Structure of Arabinosyltransferase EmbB from Mycobacterium smegmatis

PDB ID 6x0o

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