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| | ==Refined structure of peptaibol zervamicin IIB in methanol solution from trans-hydrogen bond J couplings== | | ==Refined structure of peptaibol zervamicin IIB in methanol solution from trans-hydrogen bond J couplings== |
| - | <StructureSection load='1r9u' size='340' side='right'caption='[[1r9u]], [[NMR_Ensembles_of_Models | 24 NMR models]]' scene=''> | + | <StructureSection load='1r9u' size='340' side='right'caption='[[1r9u]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1r9u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Emericellopsis_salmosynnemata Emericellopsis salmosynnemata]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9U OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1R9U FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1r9u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Emericellopsis_salmosynnemata Emericellopsis salmosynnemata]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R9U FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=AIB:ALPHA-AMINOISOBUTYRIC+ACID'>AIB</scene>, <scene name='pdbligand=DIV:D-ISOVALINE'>DIV</scene>, <scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene>, <scene name='pdbligand=PHL:L-PHENYLALANINOL'>PHL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 24 models</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1m24|1m24]], [[1dlz|1dlz]], [[1ih9|1ih9]], [[1gq0|1gq0]], [[1joh|1joh]], [[1amt|1amt]], [[1ee7|1ee7]], [[1ob7|1ob7]], [[1ob6|1ob6]], [[1ob4|1ob4]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=AIB:ALPHA-AMINOISOBUTYRIC+ACID'>AIB</scene>, <scene name='pdbligand=DIV:D-ISOVALINE'>DIV</scene>, <scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene>, <scene name='pdbligand=PHL:L-PHENYLALANINOL'>PHL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1r9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9u OCA], [http://pdbe.org/1r9u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1r9u RCSB], [http://www.ebi.ac.uk/pdbsum/1r9u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1r9u ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9u OCA], [https://pdbe.org/1r9u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r9u RCSB], [https://www.ebi.ac.uk/pdbsum/1r9u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r9u ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | [[Category: Emericellopsis salmosynnemata]] | | [[Category: Emericellopsis salmosynnemata]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Arseniev, A S]] | + | [[Category: Arseniev AS]] |
| - | [[Category: Balashova, T A]] | + | [[Category: Balashova TA]] |
| - | [[Category: Ovchinnikova, T V]] | + | [[Category: Ovchinnikova TV]] |
| - | [[Category: Shenkarev, Z O]] | + | [[Category: Shenkarev ZO]] |
| - | [[Category: Yakimenko, Z A]] | + | [[Category: Yakimenko ZA]] |
| - | [[Category: Antibacterial]]
| + | |
| - | [[Category: Antibiotic]]
| + | |
| - | [[Category: Antifungal]]
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| - | [[Category: Bent helix]]
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| - | [[Category: Bifurcated hydrogen bond]]
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| - | [[Category: Peptaibol]]
| + | |
| - | [[Category: Zrevamicin]]
| + | |
| Structural highlights
1r9u is a 1 chain structure with sequence from Emericellopsis salmosynnemata. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | Solution NMR, 24 models |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Publication Abstract from PubMed
Zervamicin IIB is a member of the alpha-aminoisobutyric acid containing peptaibol antibiotics. A new procedure for the biosynthetic preparation of the uniformly 13C- and 15N-enriched peptaibol is described This compound was isolated from the biomass of the fungus-producer Emericellopsis salmosynnemata strain 336 IMI 58330 obtained upon cultivation in the totally 13C, 15N-labelled complete medium. To prepare such a medium the autolysed biomass and the exopolysaccharides of the obligate methylotrophic bacterium Methylobacillus flagellatus KT were used. This microorganism was grown in totally 13C, 15N-labelled minimal medium containing 13C-methanol and 15N-ammonium chloride as the only carbon and nitrogen sources. Preliminary NMR spectroscopic analysis indicated a high extent of isotope incorporation (> 90%) and led to the complete 13C- and 15N-NMR assignment including the stereospecific assignment of Aib residues methyl groups. The observed pattern of the structurally important secondary chemical shifts of 1H(alpha), 13C=O and 13C(alpha) agrees well with the previously determined structure of zervamicin IIB in methanol solution.
Biosynthetic uniform 13C,15N-labelling of zervamicin IIB. Complete 13C and 15N NMR assignment.,Ovchinnikova TV, Shenkarev ZO, Yakimenko ZA, Svishcheva NV, Tagaev AA, Skladnev DA, Arseniev AS J Pept Sci. 2003 Nov-Dec;9(11-12):817-26. PMID:14658801[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ovchinnikova TV, Shenkarev ZO, Yakimenko ZA, Svishcheva NV, Tagaev AA, Skladnev DA, Arseniev AS. Biosynthetic uniform 13C,15N-labelling of zervamicin IIB. Complete 13C and 15N NMR assignment. J Pept Sci. 2003 Nov-Dec;9(11-12):817-26. PMID:14658801 doi:10.1002/psc.499
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