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2xr1

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DIMERIC ARCHAEAL CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR WITH N-TERMINAL KH DOMAINS (KH-CPSF) FROM METHANOSARCINA MAZEI

Structural highlights

2xr1 is a 2 chain structure with sequence from Methanosarcina mazei. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.59Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8PZ03_METMA

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Proteins of the metallo-beta-lactamase (MbetaL) fold form a large superfamily of metallo-hydrolase/oxidoreductases. Members of this family are found in all three domains of life and are involved in a variety of biological functions related to hydrolysis, redox processes, DNA repair and uptake, and RNA processing. We classified the archaeal homologs of this superfamily based on sequence similarity and characterized a subfamily of the Cleavage and Polyadenylation Specificity Factor (CPSF) with an uncommon domain composition: in addition to an extended MbetaL domain, which accommodates the active site for RNA cleavage, this group has two N-terminal KH domains. Here, we present the crystal structure of a member of this group from Methanosarcina mazei. It reveals a dimerization mode of the MbetaL domain that has not been observed before and suggests that RNA is bound across the dimer interface, recognized by the KH domains of one monomer, and cleaved at the active site of the other.

Crystal structure of a dimeric archaeal Cleavage and Polyadenylation Specificity Factor.,Mir-Montazeri B, Ammelburg M, Forouzan D, Lupas AN, Hartmann MD J Struct Biol. 2010 Sep 17. PMID:20851187^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Mir-Montazeri B, Ammelburg M, Forouzan D, Lupas AN, Hartmann MD. Crystal structure of a dimeric archaeal Cleavage and Polyadenylation Specificity Factor. J Struct Biol. 2010 Sep 17. PMID:20851187 doi:10.1016/j.jsb.2010.09.013

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2xr1"

@@ Line 3: / Line 3: @@
 <StructureSection load='2xr1' size='340' side='right'caption='[[2xr1]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2xr1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_2053 Dsm 2053]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XR1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XR1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2xr1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_mazei Methanosarcina mazei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XR1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XR1 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.59&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xr1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xr1 OCA], [http://pdbe.org/2xr1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xr1 RCSB], [http://www.ebi.ac.uk/pdbsum/2xr1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xr1 ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xr1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xr1 OCA], [https://pdbe.org/2xr1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xr1 RCSB], [https://www.ebi.ac.uk/pdbsum/2xr1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xr1 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8PZ03_METMA Q8PZ03_METMA]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 30: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Dsm 2053]]
 [[Category: Large Structures]]
-[[Category: Ammelburg, M]]
+[[Category: Methanosarcina mazei]]
-[[Category: Forouzan, D]]
+[[Category: Ammelburg M]]
-[[Category: Hartmann, M D]]
+[[Category: Forouzan D]]
-[[Category: Lupas, A N]]
+[[Category: Hartmann MD]]
-[[Category: Mir-Montazeri, B]]
+[[Category: Lupas AN]]
-[[Category: Beta-casp]]
+[[Category: Mir-Montazeri B]]
-[[Category: Hydrolase]]
-[[Category: Metallo-beta-lactamase]]
-[[Category: Rna processing]]

2xr1

From Proteopedia

Current revision

DIMERIC ARCHAEAL CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR WITH N-TERMINAL KH DOMAINS (KH-CPSF) FROM METHANOSARCINA MAZEI

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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