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| | <StructureSection load='2xtb' size='340' side='right'caption='[[2xtb]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='2xtb' size='340' side='right'caption='[[2xtb]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2xtb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trybr Trybr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XTB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XTB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2xtb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_rhodesiense Trypanosoma brucei rhodesiense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XTB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XTB FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KRM:4-[5-(4-PHENOXYPHENYL)-1H-PYRAZOL-3-YL]MORPHOLINE'>KRM</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenosine_kinase Adenosine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.20 2.7.1.20] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KRM:4-[5-(4-PHENOXYPHENYL)-1H-PYRAZOL-3-YL]MORPHOLINE'>KRM</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xtb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xtb OCA], [http://pdbe.org/2xtb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xtb RCSB], [http://www.ebi.ac.uk/pdbsum/2xtb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xtb ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xtb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xtb OCA], [https://pdbe.org/2xtb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xtb RCSB], [https://www.ebi.ac.uk/pdbsum/2xtb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xtb ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q584S0_TRYB2 Q584S0_TRYB2] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Adenosine kinase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Trybr]] | + | [[Category: Trypanosoma brucei rhodesiense]] |
| - | [[Category: Ahmed, S]] | + | [[Category: Ahmed S]] |
| - | [[Category: Greenwald, J]] | + | [[Category: Greenwald J]] |
| - | [[Category: Kostrewa, D]] | + | [[Category: Kostrewa D]] |
| - | [[Category: Kuettel, S]] | + | [[Category: Kuettel S]] |
| - | [[Category: Perozzo, R]] | + | [[Category: Perozzo R]] |
| - | [[Category: Scapozza, L]] | + | [[Category: Scapozza L]] |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
Q584S0_TRYB2
Publication Abstract from PubMed
BACKGROUND: The essential purine salvage pathway of Trypanosoma brucei bears interesting catalytic enzymes for chemotherapeutic intervention of Human African Trypanosomiasis. Unlike mammalian cells, trypanosomes lack de novo purine synthesis and completely rely on salvage from their hosts. One of the key enzymes is adenosine kinase which catalyzes the phosphorylation of ingested adenosine to form adenosine monophosphate (AMP) utilizing adenosine triphosphate (ATP) as the preferred phosphoryl donor. METHODS AND FINDINGS: Here, we present the first structures of Trypanosoma brucei rhodesiense adenosine kinase (TbrAK): the structure of TbrAK in complex with the bisubstrate inhibitor P(1),P(5)-di(adenosine-5')-pentaphosphate (AP5A) at 1.55 A, and TbrAK complexed with the recently discovered activator 4-[5-(4-phenoxyphenyl)-2H-pyrazol-3-yl]morpholine (compound 1) at 2.8 A resolution. CONCLUSIONS: The structural details and their comparison give new insights into substrate and activator binding to TbrAK at the molecular level. Further structure-activity relationship analyses of a series of derivatives of compound 1 support the observed binding mode of the activator and provide a possible mechanism of action with respect to their activating effect towards TbrAK.
Crystal Structures of T. b. rhodesiense Adenosine Kinase Complexed with Inhibitor and Activator: Implications for Catalysis and Hyperactivation.,Kuettel S, Greenwald J, Kostrewa D, Ahmed S, Scapozza L, Perozzo R PLoS Negl Trop Dis. 2011 May;5(5):e1164. Epub 2011 May 24. PMID:21629723[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kuettel S, Greenwald J, Kostrewa D, Ahmed S, Scapozza L, Perozzo R. Crystal Structures of T. b. rhodesiense Adenosine Kinase Complexed with Inhibitor and Activator: Implications for Catalysis and Hyperactivation. PLoS Negl Trop Dis. 2011 May;5(5):e1164. Epub 2011 May 24. PMID:21629723 doi:10.1371/journal.pntd.0001164
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