|
|
| (One intermediate revision not shown.) |
| Line 3: |
Line 3: |
| | <StructureSection load='2xub' size='340' side='right'caption='[[2xub]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='2xub' size='340' side='right'caption='[[2xub]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2xub]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XUB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XUB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2xub]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XUB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XUB FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xv4|2xv4]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xub OCA], [http://pdbe.org/2xub PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xub RCSB], [http://www.ebi.ac.uk/pdbsum/2xub PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xub ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xub OCA], [https://pdbe.org/2xub PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xub RCSB], [https://www.ebi.ac.uk/pdbsum/2xub PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xub ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/RPC3_HUMAN RPC3_HUMAN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 25: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Fribourg, S]] | + | [[Category: Fribourg S]] |
| - | [[Category: Lefevre, S]] | + | [[Category: Lefevre S]] |
| - | [[Category: Legrand, P]] | + | [[Category: Legrand P]] |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Winged helix]]
| + | |
| Structural highlights
Function
RPC3_HUMAN
Publication Abstract from PubMed
The 17-subunit human RNA polymerase III (hPol III) transcribes small, untranslated RNA genes that are involved in the regulation of transcription, splicing and translation. hPol III subunits hRPC62, hRPC39 and hRPC32 form a stable ternary subcomplex required for promoter-specific transcription initiation by hPol III. Here, we report the crystal structure of hRPC62. This subunit folds as a four-tandem extended winged helix (eWH) protein that is structurally related to the transcription factor TFIIEalpha N terminus. Through biochemical analyses, we mapped the protein-protein interactions of hRPC62, hRPC32 and hRPC39. In addition, we demonstrated that hRPC62 and hRPC39 bind single-stranded and duplex DNA, respectively, in a sequence-independent manner. Overall, we shed light on structural similarities between the hPol III-specific subunit hRPC62 and TFIIEalpha and propose specific functions for hRPC39 and hRPC62 in transcription initiation by hPol III.
Structure-function analysis of hRPC62 provides insights into RNA polymerase III transcription initiation.,Lefevre S, Dumay-Odelot H, El-Ayoubi L, Budd A, Legrand P, Pinaud N, Teichmann M, Fribourg S Nat Struct Mol Biol. 2011 Mar;18(3):352-8. Epub 2011 Feb 27. PMID:21358628[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Lefevre S, Dumay-Odelot H, El-Ayoubi L, Budd A, Legrand P, Pinaud N, Teichmann M, Fribourg S. Structure-function analysis of hRPC62 provides insights into RNA polymerase III transcription initiation. Nat Struct Mol Biol. 2011 Mar;18(3):352-8. Epub 2011 Feb 27. PMID:21358628 doi:10.1038/nsmb.1996
|
