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2xv4

From Proteopedia

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Structure of Human RPC62 (partial)

Structural highlights

2xv4 is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.95Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RPC3_HUMAN

Publication Abstract from PubMed

The 17-subunit human RNA polymerase III (hPol III) transcribes small, untranslated RNA genes that are involved in the regulation of transcription, splicing and translation. hPol III subunits hRPC62, hRPC39 and hRPC32 form a stable ternary subcomplex required for promoter-specific transcription initiation by hPol III. Here, we report the crystal structure of hRPC62. This subunit folds as a four-tandem extended winged helix (eWH) protein that is structurally related to the transcription factor TFIIEalpha N terminus. Through biochemical analyses, we mapped the protein-protein interactions of hRPC62, hRPC32 and hRPC39. In addition, we demonstrated that hRPC62 and hRPC39 bind single-stranded and duplex DNA, respectively, in a sequence-independent manner. Overall, we shed light on structural similarities between the hPol III-specific subunit hRPC62 and TFIIEalpha and propose specific functions for hRPC39 and hRPC62 in transcription initiation by hPol III.

Structure-function analysis of hRPC62 provides insights into RNA polymerase III transcription initiation.,Lefevre S, Dumay-Odelot H, El-Ayoubi L, Budd A, Legrand P, Pinaud N, Teichmann M, Fribourg S Nat Struct Mol Biol. 2011 Mar;18(3):352-8. Epub 2011 Feb 27. PMID:21358628^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lefevre S, Dumay-Odelot H, El-Ayoubi L, Budd A, Legrand P, Pinaud N, Teichmann M, Fribourg S. Structure-function analysis of hRPC62 provides insights into RNA polymerase III transcription initiation. Nat Struct Mol Biol. 2011 Mar;18(3):352-8. Epub 2011 Feb 27. PMID:21358628 doi:10.1038/nsmb.1996

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2xv4"

Categories: Homo sapiens | Large Structures | Fribourg S | Lefevre S | Legrand P

@@ Line 3: / Line 3: @@
 <StructureSection load='2xv4' size='340' side='right'caption='[[2xv4]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2xv4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XV4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XV4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2xv4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XV4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XV4 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xub|2xub]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xv4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xv4 OCA], [http://pdbe.org/2xv4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xv4 RCSB], [http://www.ebi.ac.uk/pdbsum/2xv4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xv4 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xv4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xv4 OCA], [https://pdbe.org/2xv4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xv4 RCSB], [https://www.ebi.ac.uk/pdbsum/2xv4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xv4 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/RPC3_HUMAN RPC3_HUMAN]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Fribourg, S]]
+[[Category: Fribourg S]]
-[[Category: Lefevre, S]]
+[[Category: Lefevre S]]
-[[Category: Legrand, P]]
+[[Category: Legrand P]]
-[[Category: Transcription]]
-[[Category: Winged helix]]

2xv4

From Proteopedia

Current revision

Structure of Human RPC62 (partial)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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