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| | <StructureSection load='2xzb' size='340' side='right'caption='[[2xzb]], [[Resolution|resolution]] 7.00Å' scene=''> | | <StructureSection load='2xzb' size='340' side='right'caption='[[2xzb]], [[Resolution|resolution]] 7.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2xzb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XZB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XZB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2xzb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XZB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XZB FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3a3y|3a3y]], [[1iwc|1iwc]], [[1iwf|1iwf]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 7Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrogen/potassium-exchanging_ATPase Hydrogen/potassium-exchanging ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.10 3.6.3.10] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xzb OCA], [https://pdbe.org/2xzb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xzb RCSB], [https://www.ebi.ac.uk/pdbsum/2xzb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xzb ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xzb OCA], [http://pdbe.org/2xzb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xzb RCSB], [http://www.ebi.ac.uk/pdbsum/2xzb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xzb ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | == Disease == | |
| - | [[http://www.uniprot.org/uniprot/ATP4B_PIG ATP4B_PIG]] Note=Parietal cell autoantigen associated with autoimmune gastritis. | |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ATP4A_PIG ATP4A_PIG]] Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for acid production in the stomach. | + | [https://www.uniprot.org/uniprot/ATP4A_PIG ATP4A_PIG] Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for acid production in the stomach. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Hydrogen/potassium-exchanging ATPase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Sus scrofa]] | | [[Category: Sus scrofa]] |
| - | [[Category: Abe, K]] | + | [[Category: Abe K]] |
| - | [[Category: Fujiyoshi, Y]] | + | [[Category: Fujiyoshi Y]] |
| - | [[Category: Tani, K]] | + | [[Category: Tani K]] |
| - | [[Category: Acid suppressant]]
| + | |
| - | [[Category: Atp-binding]]
| + | |
| - | [[Category: Beryllium fluoride]]
| + | |
| - | [[Category: H/k-atpase]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Ion pump]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: P-type atpase]]
| + | |
| Structural highlights
Function
ATP4A_PIG Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for acid production in the stomach.
Publication Abstract from PubMed
Acid-related gastric diseases are associated with disorder of digestive tract acidification. The gastric proton pump, H(+),K(+)-ATPase, exports H(+) in exchange for luminal K(+) to generate a highly acidic environment in the stomach, and is a main target for acid suppressants. Here, we report the three-dimensional structure of gastric H(+),K(+)-ATPase with bound SCH28080, a representative K(+)-competitive acid blocker, at 7 A resolution based on electron crystallography of two-dimensional crystals. The density of the bound SCH28080 is found near transmembrane (TM) helices 4, 5 and 6, in the luminal cavity. The SCH28080-binding site is formed by the rearrangement of TM helices, which is in turn transmitted to the cytoplasmic domains, resulting in a luminal-open conformation. These results represent the first structural evidence for a binding site of an acid suppressant on H(+),K(+)-ATPase, and the conformational change induced by this class of drugs.
Conformational rearrangement of gastric H(+),K(+)-ATPase induced by an acid suppressant.,Abe K, Tani K, Fujiyoshi Y Nat Commun. 2011 Jan;2(1):155. PMID:21224846[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Abe K, Tani K, Fujiyoshi Y. Conformational rearrangement of gastric H(+),K(+)-ATPase induced by an acid suppressant. Nat Commun. 2011 Jan;2(1):155. PMID:21224846 doi:10.1038/ncomms1154
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