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2y20

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The mechanisms of HAMP-mediated signaling in transmembrane receptors - the A291I mutant

Structural highlights

2y20 is a 6 chain structure with sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.65Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O28769_ARCFU

Publication Abstract from PubMed

HAMP domains mediate signal transduction in over 7500 enzyme-coupled receptors represented in all kingdoms of life. The HAMP domain of the putative archaeal receptor Af1503 has a parallel, dimeric, four-helical coiled coil structure, but with unusual core packing, related to canonical packing by concerted axial rotation of the helices. This has led to the gearbox model for signal transduction, whereby the alternate packing modes correspond to signaling states. Here we present structures of a series of Af1503 HAMP variants. We show that substitution of a conserved small side chain within the domain core (A291) for larger residues induces a gradual transition in packing mode, involving both changes in helix rotation and bundle shape, which are most prominent at the C-terminal, output end of the domain. These are correlated with activity and ligand response in vitro and in vivo by incorporating Af1503 HAMP into mycobacterial adenylyl cyclase assay systems.

The Mechanisms of HAMP-Mediated Signaling in Transmembrane Receptors.,Ferris HU, Dunin-Horkawicz S, Mondejar LG, Hulko M, Hantke K, Martin J, Schultz JE, Zeth K, Lupas AN, Coles M Structure. 2011 Mar 9;19(3):378-85. PMID:21397188^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ferris HU, Dunin-Horkawicz S, Mondejar LG, Hulko M, Hantke K, Martin J, Schultz JE, Zeth K, Lupas AN, Coles M. The Mechanisms of HAMP-Mediated Signaling in Transmembrane Receptors. Structure. 2011 Mar 9;19(3):378-85. PMID:21397188 doi:10.1016/j.str.2011.01.006

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2y20"

@@ Line 3: / Line 3: @@
 <StructureSection load='2y20' size='340' side='right'caption='[[2y20]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2y20]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Arcfl Arcfl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y20 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2Y20 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2y20]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y20 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y20 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2y0q|2y0q]], [[3zrx|3zrx]], [[2y21|2y21]], [[3zrv|3zrv]], [[3zrw|3zrw]], [[2y0t|2y0t]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2y20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y20 OCA], [http://pdbe.org/2y20 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2y20 RCSB], [http://www.ebi.ac.uk/pdbsum/2y20 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2y20 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y20 OCA], [https://pdbe.org/2y20 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y20 RCSB], [https://www.ebi.ac.uk/pdbsum/2y20 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y20 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/O28769_ARCFU O28769_ARCFU]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Arcfl]]
+[[Category: Archaeoglobus fulgidus]]
 [[Category: Large Structures]]
-[[Category: Ferris, H U]]
+[[Category: Ferris HU]]
-[[Category: Hulko, M]]
+[[Category: Hulko M]]
-[[Category: Lupas, A N]]
+[[Category: Lupas AN]]
-[[Category: Zeth, K]]
+[[Category: Zeth K]]
-[[Category: Membrane protein]]
-[[Category: Transmembrane signalling]]

2y20

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Current revision

The mechanisms of HAMP-mediated signaling in transmembrane receptors - the A291I mutant

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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