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| | <StructureSection load='2y9z' size='340' side='right'caption='[[2y9z]], [[Resolution|resolution]] 3.60Å' scene=''> | | <StructureSection load='2y9z' size='340' side='right'caption='[[2y9z]], [[Resolution|resolution]] 3.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2y9z]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y9Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2Y9Z FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2y9z]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y9Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y9Z FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2y9y|2y9y]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.601Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2y9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y9z OCA], [http://pdbe.org/2y9z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2y9z RCSB], [http://www.ebi.ac.uk/pdbsum/2y9z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2y9z ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y9z OCA], [https://pdbe.org/2y9z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y9z RCSB], [https://www.ebi.ac.uk/pdbsum/2y9z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y9z ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ISW1_YEAST ISW1_YEAST]] Catalytic component of ISW1-type complexes, which act by remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. They are involved in coordinating transcriptional repression, activation and elongation phases. The ISW1A complex represses gene expression at initiation through specific positioning of a promoter proximal dinucleosome. The ISW1B complex acts within coding regions to control the amount of RNA polymerase II released into productive elongation and to coordinate elongation with termination and pre-mRNA processing.<ref>PMID:10090725</ref> <ref>PMID:11238381</ref> <ref>PMID:14622597</ref> <ref>PMID:12482963</ref> [[http://www.uniprot.org/uniprot/IOC3_YEAST IOC3_YEAST]] Functions as component of the ISW1A complex, which acts in remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. The ISW1A complex represses gene expression at initiation through specific positioning of a promoter proximal dinucleosome.<ref>PMID:14622597</ref> <ref>PMID:12482963</ref> | + | [https://www.uniprot.org/uniprot/ISW1_YEAST ISW1_YEAST] Catalytic component of ISW1-type complexes, which act by remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. They are involved in coordinating transcriptional repression, activation and elongation phases. The ISW1A complex represses gene expression at initiation through specific positioning of a promoter proximal dinucleosome. The ISW1B complex acts within coding regions to control the amount of RNA polymerase II released into productive elongation and to coordinate elongation with termination and pre-mRNA processing.<ref>PMID:10090725</ref> <ref>PMID:11238381</ref> <ref>PMID:14622597</ref> <ref>PMID:12482963</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Angst, B]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: DeLuca, C]] | + | [[Category: Angst B]] |
| - | [[Category: Fitzgerald, D J]] | + | [[Category: DeLuca C]] |
| - | [[Category: Frouws, T D]] | + | [[Category: Fitzgerald DJ]] |
| - | [[Category: Richmond, T J]] | + | [[Category: Frouws TD]] |
| - | [[Category: Sargent, D F]] | + | [[Category: Richmond TJ]] |
| - | [[Category: Schimmele, K]] | + | [[Category: Sargent DF]] |
| - | [[Category: Yamada, K]] | + | [[Category: Schimmele K]] |
| - | [[Category: Chromatin remodeling]]
| + | [[Category: Yamada K]] |
| - | [[Category: Nuclear protein-dna complex]]
| + | |
| - | [[Category: Nucleosome remodeling]]
| + | |
| - | [[Category: Transcription]]
| + | |
| Structural highlights
Function
ISW1_YEAST Catalytic component of ISW1-type complexes, which act by remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. They are involved in coordinating transcriptional repression, activation and elongation phases. The ISW1A complex represses gene expression at initiation through specific positioning of a promoter proximal dinucleosome. The ISW1B complex acts within coding regions to control the amount of RNA polymerase II released into productive elongation and to coordinate elongation with termination and pre-mRNA processing.[1] [2] [3] [4]
Publication Abstract from PubMed
Site-specific recognition of DNA in eukaryotic organisms depends on the arrangement of nucleosomes in chromatin. In the yeast Saccharomyces cerevisiae, ISW1a and related chromatin remodelling factors are implicated in establishing the nucleosome repeat during replication and altering nucleosome position to affect gene activity. Here we have solved the crystal structures of S. cerevisiae ISW1a lacking its ATPase domain both alone and with DNA bound at resolutions of 3.25 A and 3.60 A, respectively, and we have visualized two different nucleosome-containing remodelling complexes using cryo-electron microscopy. The composite X-ray and electron microscopy structures combined with site-directed photocrosslinking analyses of these complexes suggest that ISW1a uses a dinucleosome substrate for chromatin remodelling. Results from a remodelling assay corroborate the dinucleosome model. We show how a chromatin remodelling factor could set the spacing between two adjacent nucleosomes acting as a 'protein ruler'.
Structure and mechanism of the chromatin remodelling factor ISW1a.,Yamada K, Frouws TD, Angst B, Fitzgerald DJ, DeLuca C, Schimmele K, Sargent DF, Richmond TJ Nature. 2011 Apr 28;472(7344):448-53. PMID:21525927[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Tsukiyama T, Palmer J, Landel CC, Shiloach J, Wu C. Characterization of the imitation switch subfamily of ATP-dependent chromatin-remodeling factors in Saccharomyces cerevisiae. Genes Dev. 1999 Mar 15;13(6):686-97. PMID:10090725
- ↑ Kent NA, Karabetsou N, Politis PK, Mellor J. In vivo chromatin remodeling by yeast ISWI homologs Isw1p and Isw2p. Genes Dev. 2001 Mar 1;15(5):619-26. PMID:11238381 doi:http://dx.doi.org/10.1101/gad.190301
- ↑ Morillon A, Karabetsou N, O'Sullivan J, Kent N, Proudfoot N, Mellor J. Isw1 chromatin remodeling ATPase coordinates transcription elongation and termination by RNA polymerase II. Cell. 2003 Nov 14;115(4):425-35. PMID:14622597
- ↑ Vary JC Jr, Gangaraju VK, Qin J, Landel CC, Kooperberg C, Bartholomew B, Tsukiyama T. Yeast Isw1p forms two separable complexes in vivo. Mol Cell Biol. 2003 Jan;23(1):80-91. PMID:12482963
- ↑ Yamada K, Frouws TD, Angst B, Fitzgerald DJ, DeLuca C, Schimmele K, Sargent DF, Richmond TJ. Structure and mechanism of the chromatin remodelling factor ISW1a. Nature. 2011 Apr 28;472(7344):448-53. PMID:21525927 doi:10.1038/nature09947
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