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| | <StructureSection load='2ygb' size='340' side='right'caption='[[2ygb]], [[Resolution|resolution]] 2.81Å' scene=''> | | <StructureSection load='2ygb' size='340' side='right'caption='[[2ygb]], [[Resolution|resolution]] 2.81Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2ygb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Vaccinia_virus_western_reserve Vaccinia virus western reserve]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YGB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2YGB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2ygb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus_Western_Reserve Vaccinia virus Western Reserve]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YGB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YGB FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2ygb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ygb OCA], [http://pdbe.org/2ygb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ygb RCSB], [http://www.ebi.ac.uk/pdbsum/2ygb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ygb ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.81Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ygb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ygb OCA], [https://pdbe.org/2ygb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ygb RCSB], [https://www.ebi.ac.uk/pdbsum/2ygb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ygb ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/D13_VACCW D13_VACCW]] Scaffold protein which forms a transitory spherical honeycomb lattice providing curvature and rigidity to the convex membrane of crescent and immature virions (IV). This association occurs concomitantly with viral membrane formation. Targeted by the drug rifampicin, which prevents the formation of this lattice, and hence virus morphogenesis. In the presence of rifampicin, irregularly shaped membranes that lack the honeycomb layer accumulate around areas of electron-dense viroplasm. This layer is lost from virions during maturation from IV to mature virion (MV), through the proteolysis of A17 N-terminus. | + | [https://www.uniprot.org/uniprot/D13_VACCW D13_VACCW] Scaffold protein which forms a transitory spherical honeycomb lattice providing curvature and rigidity to the convex membrane of crescent and immature virions (IV). This association occurs concomitantly with viral membrane formation. Targeted by the drug rifampicin, which prevents the formation of this lattice, and hence virus morphogenesis. In the presence of rifampicin, irregularly shaped membranes that lack the honeycomb layer accumulate around areas of electron-dense viroplasm. This layer is lost from virions during maturation from IV to mature virion (MV), through the proteolysis of A17 N-terminus. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Vaccinia virus western reserve]] | + | [[Category: Vaccinia virus Western Reserve]] |
| - | [[Category: Bahar, M W]] | + | [[Category: Bahar MW]] |
| - | [[Category: Graham, S C]] | + | [[Category: Graham SC]] |
| - | [[Category: Grimes, J M]] | + | [[Category: Grimes JM]] |
| - | [[Category: Stuart, D I]] | + | [[Category: Stuart DI]] |
| - | [[Category: Viral evolution]]
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| - | [[Category: Viral protein]]
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| Structural highlights
Function
D13_VACCW Scaffold protein which forms a transitory spherical honeycomb lattice providing curvature and rigidity to the convex membrane of crescent and immature virions (IV). This association occurs concomitantly with viral membrane formation. Targeted by the drug rifampicin, which prevents the formation of this lattice, and hence virus morphogenesis. In the presence of rifampicin, irregularly shaped membranes that lack the honeycomb layer accumulate around areas of electron-dense viroplasm. This layer is lost from virions during maturation from IV to mature virion (MV), through the proteolysis of A17 N-terminus.
Publication Abstract from PubMed
The morphogenesis of poxviruses such as vaccinia virus (VACV) sees the virion shape mature from spherical to brick-shaped. Trimeric capsomers of the VACV D13 protein form a transitory, stabilizing lattice on the surface of the initial spherical immature virus particle. The crystal structure of D13 reveals that this major scaffolding protein comprises a double beta barrel "jelly-roll" subunit arranged as pseudo-hexagonal trimers. These structural features are characteristic of the major capsid proteins of a lineage of large icosahedral double-stranded DNA viruses including human adenovirus and the bacteriophages PRD1 and PM2. Structure-based phylogenetic analysis confirms that VACV belongs to this lineage, suggesting that (analogously to higher organism embryogenesis) early poxvirus morphogenesis reflects their evolution from a lineage of viruses sharing a common icosahedral ancestor.
Insights into the evolution of a complex virus from the crystal structure of vaccinia virus d13.,Bahar MW, Graham SC, Stuart DI, Grimes JM Structure. 2011 Jul 13;19(7):1011-20. PMID:21742267[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bahar MW, Graham SC, Stuart DI, Grimes JM. Insights into the evolution of a complex virus from the crystal structure of vaccinia virus d13. Structure. 2011 Jul 13;19(7):1011-20. PMID:21742267 doi:10.1016/j.str.2011.03.023
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