1c8b

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[[Image:1c8b.gif|left|200px]]
 
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==CRYSTAL STRUCTURE OF A NOVEL GERMINATION PROTEASE FROM SPORES OF BACILLUS MEGATERIUM: STRUCTURAL REARRANGEMENTS AND ZYMOGEN ACTIVATION==
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The line below this paragraph, containing "STRUCTURE_1c8b", creates the "Structure Box" on the page.
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<StructureSection load='1c8b' size='340' side='right'caption='[[1c8b]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1c8b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Priestia_megaterium Priestia megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C8B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C8B FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c8b OCA], [https://pdbe.org/1c8b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c8b RCSB], [https://www.ebi.ac.uk/pdbsum/1c8b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c8b ProSAT]</span></td></tr>
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{{STRUCTURE_1c8b| PDB=1c8b | SCENE= }}
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</table>
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== Function ==
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'''CRYSTAL STRUCTURE OF A NOVEL GERMINATION PROTEASE FROM SPORES OF BACILLUS MEGATERIUM: STRUCTURAL REARRANGEMENTS AND ZYMOGEN ACTIVATION'''
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[https://www.uniprot.org/uniprot/GPR_PRIM1 GPR_PRIM1] Initiates the rapid degradation of small, acid-soluble proteins during spore germination.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c8/1c8b_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1c8b ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
The DNA in the core of spores of Bacillus species is saturated with a group of small, acid-soluble proteins (SASP) that protect DNA from a variety of harsh treatments and play a major role in spore resistance and long-term spore survival. During spore germination, SASPs are rapidly degraded to amino acids and this degradation is initiated by a sequence-specific protease called germination protease (GPR), which exhibits no obvious mechanistic or amino acid sequence similarity to any known class of proteases. GPR is synthesized during sporulation as an inactive tetrameric zymogen termed P(46), which later autoprocesses to a smaller form termed P(41), which is active only during spore germination. Here, we report the crystal structure of P(46) from Bacillus megaterium at 3.0 A resolution and the fact that P(46) monomer adopts a novel fold. The asymmetric unit contains two P(46) monomers and the functional tetramer is a dimer of dimers, with an approximately 9 A channel in the center of the tetramer. Analysis of the P(46) structure and site-directed mutagenesis studies have provided some insight into the mechanism of zymogen activation as well as the zymogen's lack of activity and the inactivity of P(41) in the mature spore.
The DNA in the core of spores of Bacillus species is saturated with a group of small, acid-soluble proteins (SASP) that protect DNA from a variety of harsh treatments and play a major role in spore resistance and long-term spore survival. During spore germination, SASPs are rapidly degraded to amino acids and this degradation is initiated by a sequence-specific protease called germination protease (GPR), which exhibits no obvious mechanistic or amino acid sequence similarity to any known class of proteases. GPR is synthesized during sporulation as an inactive tetrameric zymogen termed P(46), which later autoprocesses to a smaller form termed P(41), which is active only during spore germination. Here, we report the crystal structure of P(46) from Bacillus megaterium at 3.0 A resolution and the fact that P(46) monomer adopts a novel fold. The asymmetric unit contains two P(46) monomers and the functional tetramer is a dimer of dimers, with an approximately 9 A channel in the center of the tetramer. Analysis of the P(46) structure and site-directed mutagenesis studies have provided some insight into the mechanism of zymogen activation as well as the zymogen's lack of activity and the inactivity of P(41) in the mature spore.
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==About this Structure==
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Crystal structure of a novel germination protease from spores of Bacillus megaterium: structural arrangement and zymogen activation.,Ponnuraj K, Rowland S, Nessi C, Setlow P, Jedrzejas MJ J Mol Biol. 2000 Jun 30;300(1):1-10. PMID:10864493<ref>PMID:10864493</ref>
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1C8B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C8B OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of a novel germination protease from spores of Bacillus megaterium: structural arrangement and zymogen activation., Ponnuraj K, Rowland S, Nessi C, Setlow P, Jedrzejas MJ, J Mol Biol. 2000 Jun 30;300(1):1-10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10864493 10864493]
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</div>
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[[Category: Bacillus megaterium]]
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<div class="pdbe-citations 1c8b" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Jedrzejas, M J.]]
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<references/>
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[[Category: Nessi, C.]]
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__TOC__
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[[Category: Ponnuraj, K.]]
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</StructureSection>
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[[Category: Rowland, S.]]
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[[Category: Large Structures]]
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[[Category: Setlow, P.]]
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[[Category: Priestia megaterium]]
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[[Category: Novel fold]]
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[[Category: Jedrzejas MJ]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:27:05 2008''
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[[Category: Nessi C]]
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[[Category: Ponnuraj K]]
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[[Category: Rowland S]]
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[[Category: Setlow P]]

Current revision

CRYSTAL STRUCTURE OF A NOVEL GERMINATION PROTEASE FROM SPORES OF BACILLUS MEGATERIUM: STRUCTURAL REARRANGEMENTS AND ZYMOGEN ACTIVATION

PDB ID 1c8b

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