This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2yky

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:12, 1 May 2024) (edit) (undo)
 
(2 intermediate revisions not shown.)
Line 3: Line 3:
<StructureSection load='2yky' size='340' side='right'caption='[[2yky]], [[Resolution|resolution]] 1.69&Aring;' scene=''>
<StructureSection load='2yky' size='340' side='right'caption='[[2yky]], [[Resolution|resolution]] 1.69&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[2yky]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mesorhizobium_sp._luk Mesorhizobium sp. luk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YKY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2YKY FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[2yky]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesorhizobium_sp._LUK Mesorhizobium sp. LUK]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YKY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YKY FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BNZ:BENZENE'>BNZ</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=SFE:(3S)-3-AMINO-3-PHENYLPROPANOIC+ACID'>SFE</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.69&#8491;</td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2yku|2yku]], [[2ykv|2ykv]], [[2ykx|2ykx]], [[4ao4|4ao4]]</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BNZ:BENZENE'>BNZ</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=SFE:(3S)-3-AMINO-3-PHENYLPROPANOIC+ACID'>SFE</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2yky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yky OCA], [http://pdbe.org/2yky PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2yky RCSB], [http://www.ebi.ac.uk/pdbsum/2yky PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2yky ProSAT]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yky OCA], [https://pdbe.org/2yky PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yky RCSB], [https://www.ebi.ac.uk/pdbsum/2yky PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yky ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/A3EYF7_9HYPH A3EYF7_9HYPH]
-
Chiral beta-amino acids occur as constituents of various natural and synthetic compounds with potentially useful bioactivities. The pyridoxal-5'-phosphate dependent (S)-selective transaminase from Mesorhizobium sp. LUK (MesAT) is a fold type I aminotransferase that can be used for the preparation of enantiopure beta-phenylalanine and derivatives thereof. Using X-ray crystallography, we solved structures of MesAT in complex with (S)-beta-phenylalanine, (R)-3-amino-5-methylhexanoic acid, 2-oxoglutarate, and the inhibitor 2-aminooxyacetic acid, which allowed us to unveil the basis of the amino acid specificity and enantioselectivity of this enzyme. The binding pocket of the side chain of a beta-amino acid is located on the 3'-O side of the PLP cofactor. The same binding pocket is utilized by MesAT to bind the alpha-carboxylate group of an alpha-amino acid. A beta-amino acid thus binds in a reverse orientation in the active site of MesAT as compared to an alpha-amino acid. Such a binding mode has not been reported before for any PLP-dependent aminotransferase and shows that the active site of MesAT has specifically evolved to accommodate both beta- and alpha-amino acids.
+
-
 
+
-
Structural Determinants of the beta-Selectivity of a Bacterial Aminotransferase.,Wybenga GG, Crismaru CG, Janssen DB, Dijkstra BW J Biol Chem. 2012 Jun 28. PMID:22745123<ref>PMID:22745123</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 2yky" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Mesorhizobium sp. luk]]
+
[[Category: Mesorhizobium sp. LUK]]
-
[[Category: Crismaru, C G]]
+
[[Category: Crismaru CG]]
-
[[Category: Dijkstra, B W]]
+
[[Category: Dijkstra BW]]
-
[[Category: Janssen, D B]]
+
[[Category: Janssen DB]]
-
[[Category: Wybenga, G G]]
+
[[Category: Wybenga GG]]
-
[[Category: Transferase]]
+

Current revision

Structural Determinants of the Beta-Selectivity of a Bacterial Aminotransferase

PDB ID 2yky

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2yky"
Personal tools