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6lmw
From Proteopedia
(Difference between revisions)
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==Cryo-EM structure of the CALHM chimeric construct (8-mer)== | ==Cryo-EM structure of the CALHM chimeric construct (8-mer)== | ||
| - | <StructureSection load='6lmw' size='340' side='right'caption='[[6lmw]]' scene=''> | + | <StructureSection load='6lmw' size='340' side='right'caption='[[6lmw]], [[Resolution|resolution]] 3.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LMW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LMW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6lmw]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Japanese_killifish Japanese killifish]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LMW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LMW FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lmw OCA], [http://pdbe.org/6lmw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lmw RCSB], [http://www.ebi.ac.uk/pdbsum/6lmw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lmw ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CALHM2, FAM26B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8090 Japanese killifish])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lmw OCA], [http://pdbe.org/6lmw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lmw RCSB], [http://www.ebi.ac.uk/pdbsum/6lmw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lmw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Calcium homeostasis modulator (CALHM) family proteins are Ca(2+)-regulated adenosine triphosphate (ATP)-release channels involved in neural functions including neurotransmission in gustation. Here, we present the cryo-electron microscopy (EM) structures of killifish CALHM1, human CALHM2, and Caenorhabditis elegans CLHM-1 at resolutions of 2.66, 3.4, and 3.6 A, respectively. The CALHM1 octamer structure reveals that the N-terminal helix forms the constriction site at the channel pore in the open state and modulates the ATP conductance. The CALHM2 undecamer and CLHM-1 nonamer structures show the different oligomeric stoichiometries among CALHM homologs. We further report the cryo-EM structures of the chimeric construct, revealing that the intersubunit interactions at the transmembrane domain (TMD) and the TMD-intracellular domain linker define the oligomeric stoichiometry. These findings advance our understanding of the ATP conduction and oligomerization mechanisms of CALHM channels. | ||
| + | |||
| + | Cryo-EM structures of calcium homeostasis modulator channels in diverse oligomeric assemblies.,Demura K, Kusakizako T, Shihoya W, Hiraizumi M, Nomura K, Shimada H, Yamashita K, Nishizawa T, Taruno A, Nureki O Sci Adv. 2020 Jul 17;6(29):eaba8105. doi: 10.1126/sciadv.aba8105. eCollection, 2020 Jul. PMID:32832629<ref>PMID:32832629</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6lmw" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Japanese killifish]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Demura K]] | + | [[Category: Demura, K]] |
| - | [[Category: Hiraizumi M]] | + | [[Category: Hiraizumi, M]] |
| - | [[Category: Kusakizako T]] | + | [[Category: Kusakizako, T]] |
| - | [[Category: Nishizawa T]] | + | [[Category: Nishizawa, T]] |
| - | [[Category: Nureki O]] | + | [[Category: Nureki, O]] |
| - | [[Category: Shihoya W]] | + | [[Category: Shihoya, W]] |
| - | [[Category: Shimada H]] | + | [[Category: Shimada, H]] |
| - | [[Category: Yamashita K]] | + | [[Category: Yamashita, K]] |
| + | [[Category: Channel]] | ||
| + | [[Category: Membrane protein]] | ||
Current revision
Cryo-EM structure of the CALHM chimeric construct (8-mer)
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