6lkt

<table><tr><td colspan='2'>[[6lkt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LKT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LKT FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lkt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lkt OCA], [http://pdbe.org/6lkt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lkt RCSB], [http://www.ebi.ac.uk/pdbsum/6lkt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lkt ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

A unique glycoprotein is expressed on the virus envelope of human herpesvirus 6B (HHV-6B): the complex gH/gL/gQ1/gQ2 (hereafter referred to as the HHV-6B tetramer). This tetramer recognizes a host receptor expressed on activated T cells: human CD134 (hCD134). This interaction is essential for HHV-6B entry into the susceptible cells and is a determinant for HHV-6B cell tropism. The structural mechanisms underlying this unique interaction were unknown. Herein we solved the interactions between the HHV-6B tetramer and the receptor by using their neutralizing antibodies in molecular and structural analyses. A surface plasmon resonance analysis revealed fast dissociation/association between the tetramer and hCD134, although the affinity was high (KD = 18 nM) and comparable to those for the neutralizing antibodies (anti-gQ1: 17 nM, anti-gH: 2.7 nM). A competition assay demonstrated that the anti-gQ1 antibody competed with hCD134 in the HHV-6B tetramer binding whereas the anti-gH antibody did not, indicating the direct interaction of gQ1 and hCD134. A single-particle analysis by negative-staining electron microscopy revealed the tetramer's elongated shape with a gH/gL part and extra density corresponding to gQ1/gQ2. The anti-gQ1 antibody bound to the tip of the extra density, and anti-gH antibody bound to the putative gH/gL part. These results highlight the interaction of gQ1/gQ2 in the HHV-6B tetramer with hCD134, and they demonstrate common features among viral ligands of the betaherpesvirus subfamily from a macroscopic viewpoint.

 

== References ==

[[Category: Aoshi, T]]

[[Category: Kato, T]]

[[Category: Kawabata, A]]

[[Category: Mori, Y]]

[[Category: Nishimura, M]]

[[Category: Novita, B D]]

[[Category: Poetranto, A L]]

[[Category: Tang, H]]

[[Category: Tjan, L H]]

[[Category: Wakata, A]]

[[Category: Wang, B]]

[[Category: Neutralizing antibody]]