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| | <StructureSection load='3aqx' size='340' side='right'caption='[[3aqx]], [[Resolution|resolution]] 2.05Å' scene=''> | | <StructureSection load='3aqx' size='340' side='right'caption='[[3aqx]], [[Resolution|resolution]] 2.05Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3aqx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bommo Bommo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AQX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3AQX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3aqx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AQX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AQX FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ie4|3ie4]], [[2rqe|2rqe]], [[2kha|2kha]], [[3aqy|3aqy]], [[3aqz|3aqz]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3aqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aqx OCA], [http://pdbe.org/3aqx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3aqx RCSB], [http://www.ebi.ac.uk/pdbsum/3aqx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3aqx ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3aqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aqx OCA], [https://pdbe.org/3aqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3aqx RCSB], [https://www.ebi.ac.uk/pdbsum/3aqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3aqx ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/BGBP_BOMMO BGBP_BOMMO]] Involved in the recognition of invading micro-organisms. Binds specifically to beta-1,3-glucan and activates the phenoloxidase cascade.<ref>PMID:10671539</ref> <ref>PMID:3136171</ref> | + | [https://www.uniprot.org/uniprot/BGBP_BOMMO BGBP_BOMMO] Involved in the recognition of invading micro-organisms. Binds specifically to beta-1,3-glucan and activates the phenoloxidase cascade.<ref>PMID:10671539</ref> <ref>PMID:3136171</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bommo]] | + | [[Category: Bombyx mori]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Adachi, Y]] | + | [[Category: Adachi Y]] |
| - | [[Category: Ikeda, A]] | + | [[Category: Ikeda A]] |
| - | [[Category: Kanagawa, M]] | + | [[Category: Kanagawa M]] |
| - | [[Category: Ohno, N]] | + | [[Category: Ohno N]] |
| - | [[Category: Satoh, T]] | + | [[Category: Satoh T]] |
| - | [[Category: Yamaguchi, Y]] | + | [[Category: Yamaguchi Y]] |
| - | [[Category: 3-glucan]]
| + | |
| - | [[Category: Beta-1]]
| + | |
| - | [[Category: Beta-sandwich]]
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| - | [[Category: Immune receptor]]
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| - | [[Category: Sugar binding protein]]
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| Structural highlights
Function
BGBP_BOMMO Involved in the recognition of invading micro-organisms. Binds specifically to beta-1,3-glucan and activates the phenoloxidase cascade.[1] [2]
Publication Abstract from PubMed
Innate ability to detect pathogens is achieved by pattern recognition receptors (PRRs) which recognise non-self components such as beta1,3-glucan. beta1,3-Glucans form a triple-helical structure stabilised by inter-chain hydrogen bonds. beta1,3-Glucan recognition protein (betaGRP)/gram-negative bacteria-binding protein 3 (GNBP3), one of PPRs, binds to long, structured beta1,3-glucan to initiate innate immune response. However, binding details and how specificity is achieved in such receptors remain important outstanding issues. We solved the crystal structures of the N-terminal beta1,3-glucan recognition domain of betaGRP/GNBP3 (betaGRP-N) in complex with the beta1,3-linked glucose hexamer, laminarihexaose. In the crystals, three structured laminarihexaoses simultaneously interact through six glucose residues (two from each chain) with one betaGRP-N. The spatial arrangement of the laminarihexaoses bound to betaGRP-N is almost identical to that of a beta1,3-glucan triplex. Therefore, our crystallographic structures together with site-directed mutagenesis data provide a structural basis for the unique recognition by such receptors of the triple-helix structure of beta1,3-glucan.
Structural insights into recognition of triple-helical {beta}-glucan by insect fungal receptor.,Kanagawa M, Satoh T, Ikeda A, Adachi Y, Ohno N, Yamaguchi Y J Biol Chem. 2011 Jun 22. PMID:21697086[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ochiai M, Ashida M. A pattern-recognition protein for beta-1,3-glucan. The binding domain and the cDNA cloning of beta-1,3-glucan recognition protein from the silkworm, Bombyx mori. J Biol Chem. 2000 Feb 18;275(7):4995-5002. PMID:10671539
- ↑ Ochiai M, Ashida M. Purification of a beta-1,3-glucan recognition protein in the prophenoloxidase activating system from hemolymph of the silkworm, Bombyx mori. J Biol Chem. 1988 Aug 25;263(24):12056-62. PMID:3136171
- ↑ Kanagawa M, Satoh T, Ikeda A, Adachi Y, Ohno N, Yamaguchi Y. Structural insights into recognition of triple-helical {beta}-glucan by insect fungal receptor. J Biol Chem. 2011 Jun 22. PMID:21697086 doi:10.1074/jbc.M111.256701
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