1caw

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[[Image:1caw.jpg|left|200px]]
 
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==DETERMINATION OF THREE CRYSTAL STRUCTURES OF CANAVALIN BY MOLECULAR REPLACEMENT==
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The line below this paragraph, containing "STRUCTURE_1caw", creates the "Structure Box" on the page.
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<StructureSection load='1caw' size='340' side='right'caption='[[1caw]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1caw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CAW FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1caw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1caw OCA], [https://pdbe.org/1caw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1caw RCSB], [https://www.ebi.ac.uk/pdbsum/1caw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1caw ProSAT]</span></td></tr>
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{{STRUCTURE_1caw| PDB=1caw | SCENE= }}
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</table>
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== Function ==
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'''DETERMINATION OF THREE CRYSTAL STRUCTURES OF CANAVALIN BY MOLECULAR REPLACEMENT'''
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[https://www.uniprot.org/uniprot/CANA_CANEN CANA_CANEN] Seed storage protein.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Canavalin, the major reserve protein of the jack bean, was obtained in four different crystal forms. From the structure determined by multiple isomorphous replacement in a hexagonal unit cell, the structures of three other crystals were determined by molecular replacement. In two cases, the rhombohedral and cubic crystals, placement was facilitated by coincidence of threefold molecular symmetry with crystallographic operators. In the orthorhombic crystal the canavalin trimer was the asymmetric unit. The rhombohedral, orthorhombic and cubic crystal structures were subsequently refined using a combination of several approaches with resulting R factors of 0.194, 0.185 and 0.211 at resolutions of 2.6, 2.6 and 2.3 A, respectively. Variation in the conformation of the molecule from crystal to crystal was small with an r.m.s. deviation in Calpha positions of 0.89 A. Packing is quite different among crystal forms but lattice interactions appear to play little role in the conformation of the molecule. Greatest variations in mean position are for those residues that also exhibit the greatest thermal motion. Crystal contacts in all crystals are mediated almost exclusively by hydrophilic side chains, and three to six intermolecular salt bridges per protein subunit are present in each case.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ca/1caw_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1CAW is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CAW OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1caw ConSurf].
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Determination of three crystal structures of canavalin by molecular replacement., Ko TP, Ng JD, Greenwood A, McPherson A, Acta Crystallogr D Biol Crystallogr. 1993 Sep 1;49(Pt 5):478-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299507 15299507]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Canavalia ensiformis]]
[[Category: Canavalia ensiformis]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Day, J.]]
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[[Category: Day J]]
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[[Category: Greenwood, A.]]
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[[Category: Greenwood A]]
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[[Category: Ko, T-P.]]
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[[Category: Ko T-P]]
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[[Category: McPherson, A.]]
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[[Category: McPherson A]]
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[[Category: Ng, J D.]]
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[[Category: Ng JD]]
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[[Category: Seed storage protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:32:02 2008''
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Current revision

DETERMINATION OF THREE CRYSTAL STRUCTURES OF CANAVALIN BY MOLECULAR REPLACEMENT

PDB ID 1caw

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