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7cmo
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 7cmo is ON HOLD Authors: Hu, F., Huang, Z., Li, L. Description: Crystal structure of human inorganic pyrophosphatase [[Category: Unreleased Structu...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of human inorganic pyrophosphatase== | |
| + | <StructureSection load='7cmo' size='340' side='right'caption='[[7cmo]], [[Resolution|resolution]] 3.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7cmo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CMO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CMO FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cmo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cmo OCA], [https://pdbe.org/7cmo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cmo RCSB], [https://www.ebi.ac.uk/pdbsum/7cmo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cmo ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/IPYR_HUMAN IPYR_HUMAN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Inorganic pyrophosphatase (PPase) plays an essential role in energy conservation and provides energy for many biosynthetic pathways. Here, we present two three-dimensional structures of PPase from Homo sapiens (Hu-PPase) at 2.38 A and 3.40 A in different crystallization conditions. One of the Hu-PPase structures complex of two magnesium metal ions was determined to be a monomer (Hu-PPase-mono) here, while the other one to be a dimer-dimer (Hu-PPase-dd). In each asymmetric unit of Hu-PPase-mono, there are four alpha-helices and ten beta-strands and folds as a barrel structure, and the active site contains two magnesium ions. Like PPases from many species, we found that Hu-PPase was able to undergo self-assembly. To our surprise, disruption of the self-assembly of Hu-PPase did not influence its enzymatic activity or the ability to promote cell growth. Our work uncovered that different structure forms of Hu-PPase and found that the pyrophosphatase activity of Hu-PPase is independent of its self-assembly. | ||
| - | + | Structural and biochemical characterization of inorganic pyrophosphatase from Homo sapiens.,Hu F, Huang Z, Zheng S, Wu Q, Chen Y, Lin H, Huang W, Li L Biochem Biophys Res Commun. 2020 Oct 6. pii: S0006-291X(20)31891-X. doi:, 10.1016/j.bbrc.2020.09.139. PMID:33036755<ref>PMID:33036755</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 7cmo" style="background-color:#fffaf0;"></div> |
| - | [[Category: Hu | + | |
| - | [[Category: Li | + | ==See Also== |
| + | *[[Inorganic pyrophosphatase 3D structures|Inorganic pyrophosphatase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Hu F]] | ||
| + | [[Category: Huang Z]] | ||
| + | [[Category: Li L]] | ||
Current revision
Crystal structure of human inorganic pyrophosphatase
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Categories: Homo sapiens | Large Structures | Hu F | Huang Z | Li L
