This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1lp8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="1lp8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lp8, resolution 1.65&Aring;" /> '''HIGH RESOLUTION STR...)
Current revision (07:34, 14 February 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1lp8.gif|left|200px]]<br />
 
-
<applet load="1lp8" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1lp8, resolution 1.65&Aring;" />
 
-
'''HIGH RESOLUTION STRUCTURE OF RECOMBINANT DIANTHIN ANTIVIRAL PROTEIN-POTENT ANTI-HIV AGENT'''<br />
 
-
==Overview==
+
==HIGH RESOLUTION STRUCTURE OF RECOMBINANT DIANTHIN ANTIVIRAL PROTEIN-POTENT ANTI-HIV AGENT==
-
Dianthin antiviral protein (DAP) is a naturally occurring antiviral, protein from the leaves of carnation (Dianthus caryophyllus) capable of, depurinating HIV-1 RNA and inhibiting HIV-1 replication in human, peripheral blood mononuclear cells. Escherichia coli-derived recombinant, DAP (rDAP, amino acids 1-254) was purified to homogeneity for structural, and functional studies. In the following paper the X-ray crystal structure, of rDAP as well as its complexes with cyclic AMP and adenyl-guanosine, (ApG) as substrate analogs at 1.7 A resolution are reported. Molecular, modeling studies of the interactions of DAP and the structurally similar, pokeweed antiviral protein (PAP) with a single-stranded RNA heptamer, predicted a more potent anti-HIV activity for rDAP due to its unique, surface topology and more favorable charge distribution in its 20 A-long, RNA binding active center cleft. In accordance with the predictions of the, modeling studies, rDAP was more potent than rPAP in depurinating HIV-1, RNA. To the knowledge of the authors, this is the first structural and, functional characterization of recombinant DAP.
+
<StructureSection load='1lp8' size='340' side='right'caption='[[1lp8]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1lp8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dianthus_caryophyllus Dianthus caryophyllus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LP8 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lp8 OCA], [https://pdbe.org/1lp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lp8 RCSB], [https://www.ebi.ac.uk/pdbsum/1lp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lp8 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RIP0_DIACA RIP0_DIACA] Single-chain ribosome-inactivating protein.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lp/1lp8_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lp8 ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1LP8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dianthus_caryophyllus Dianthus caryophyllus]. Active as [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LP8 OCA].
+
*[[Ribosome inactivating protein 3D structures|Ribosome inactivating protein 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
High resolution X-ray structure and potent anti-HIV activity of recombinant dianthin antiviral protein., Kurinov IV, Rajamohan F, Uckun FM, Arzneimittelforschung. 2004;54(10):692-702. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15553110 15553110]
+
[[Category: Dianthus caryophyllus]]
[[Category: Dianthus caryophyllus]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: rRNA N-glycosylase]]
+
[[Category: Kurinov IV]]
-
[[Category: Kurinov, I.V.]]
+
[[Category: Rajamohan F]]
-
[[Category: Rajamohan, F.]]
+
[[Category: Uckun FM]]
-
[[Category: Uckun, F.M.]]
+
-
[[Category: anti-hiv agent]]
+
-
[[Category: dianthin antiviral protein]]
+
-
[[Category: hiv-1 integrase inhibitor]]
+
-
[[Category: polynucleotide:adenosine glycosidase]]
+
-
[[Category: ribosome inactivating protein]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov 8 14:17:30 2007''
+

Current revision

HIGH RESOLUTION STRUCTURE OF RECOMBINANT DIANTHIN ANTIVIRAL PROTEIN-POTENT ANTI-HIV AGENT

PDB ID 1lp8

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1lp8"
Personal tools