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| - | ==Cryatal stucture of AofleA from Arthrobotrys oligospora== | + | ==Crystal structure of AofleA from Arthrobotrys oligospora== |
| | <StructureSection load='7c37' size='340' side='right'caption='[[7c37]], [[Resolution|resolution]] 1.45Å' scene=''> | | <StructureSection load='7c37' size='340' side='right'caption='[[7c37]], [[Resolution|resolution]] 1.45Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[7c37]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Artoa Artoa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C37 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7C37 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7c37]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Orbilia_oligospora_ATCC_24927 Orbilia oligospora ATCC 24927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C37 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C37 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCN:BICINE'>BCN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.451Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AOL_s00076g540 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=756982 ARTOA])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCN:BICINE'>BCN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7c37 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c37 OCA], [http://pdbe.org/7c37 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7c37 RCSB], [http://www.ebi.ac.uk/pdbsum/7c37 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7c37 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c37 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c37 OCA], [https://pdbe.org/7c37 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c37 RCSB], [https://www.ebi.ac.uk/pdbsum/7c37 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c37 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/G1XA82_ARTOA G1XA82_ARTOA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Artoa]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Cheng, X]] | + | [[Category: Orbilia oligospora ATCC 24927]] |
| - | [[Category: Liu, M]] | + | [[Category: Cheng X]] |
| - | [[Category: Wang, J]] | + | [[Category: Liu M]] |
| - | [[Category: Wang, M]] | + | [[Category: Wang J]] |
| - | [[Category: Zhang, M]] | + | [[Category: Wang M]] |
| - | [[Category: Arthrobotrys oligospora]]
| + | [[Category: Zhang M]] |
| - | [[Category: Fucose-specific lectin]]
| + | |
| - | [[Category: Nematode-trapping fungi]]
| + | |
| - | [[Category: Sugar binding protein]]
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| Structural highlights
Function
G1XA82_ARTOA
Publication Abstract from PubMed
Fungal lectin can bind specific carbohydrate structures of the host and work in recognition and adhesion or as a toxic factor. AofleA, as a fucose-specific lectin from widely studied nematode predatory fungus Arthrobotrys oligospora, possibly plays a key role in the event of capturing nematodes, but the mechanism remains unknown. Here we report the crystal structure of AofleA, which exists as a homodimer with each subunit folds as a six-bladed beta-propeller. Our structural and biological results revealed that three of the six putative binding sites of AofleA had fucose-binding abilities. In addition, we found that AofleA could bind to the pharynx and intestine of the nematode in a fucose-binding-dependent manner. Our results facilitate the understanding of the mechanism that fucose-specific lectin mediates fungi-nematodes interaction, and provide structural information for the development of potential applications of AofleA.
Structural insights into the fungi-nematodes interaction mediated by fucose-specific lectin AofleA from Arthrobotrys oligospora.,Liu M, Cheng X, Wang J, Tian D, Tang K, Xu T, Zhang M, Wang Y, Wang M Int J Biol Macromol. 2020 Jul 19;164:783-793. doi:, 10.1016/j.ijbiomac.2020.07.173. PMID:32698064[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Liu M, Cheng X, Wang J, Tian D, Tang K, Xu T, Zhang M, Wang Y, Wang M. Structural insights into the fungi-nematodes interaction mediated by fucose-specific lectin AofleA from Arthrobotrys oligospora. Int J Biol Macromol. 2020 Jul 19;164:783-793. doi:, 10.1016/j.ijbiomac.2020.07.173. PMID:32698064 doi:http://dx.doi.org/10.1016/j.ijbiomac.2020.07.173
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