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| | <StructureSection load='3bcy' size='340' side='right'caption='[[3bcy]], [[Resolution|resolution]] 1.70Å' scene=''> | | <StructureSection load='3bcy' size='340' side='right'caption='[[3bcy]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3bcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BCY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3BCY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3bcy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BCY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BCY FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3bcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bcy OCA], [http://pdbe.org/3bcy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bcy RCSB], [http://www.ebi.ac.uk/pdbsum/3bcy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bcy ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bcy OCA], [https://pdbe.org/3bcy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bcy RCSB], [https://www.ebi.ac.uk/pdbsum/3bcy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bcy ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | == Function == | |
| - | [[http://www.uniprot.org/uniprot/RGI1_YEAST RGI1_YEAST]] Involved in the control of energetic metabolism and significantly contribute to cell fitness, especially under respiratory growth conditions.<ref>PMID:20567505</ref> | |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | <jmolCheckbox> | | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bc/3bcy_consurf.spt"</scriptWhenChecked> | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bc/3bcy_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> | | </jmolCheckbox> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Gehring, K]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Kozlov, G]] | + | [[Category: Gehring K]] |
| - | [[Category: Mixed alpha-helix/beta-sheet fold]] | + | [[Category: Kozlov G]] |
| - | [[Category: Unknown function]]
| + | |
| Structural highlights
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The genome of Saccharomyces cerevisiae is arguably the best studied eukaryotic genome, and yet, it contains approximately 1000 genes that are still relatively uncharacterized. As the majority of these ORFs have no homologs with characterized sequence or protein structure, traditional sequence-based approaches cannot be applied to deduce their biological function. Here, we characterize YER067W, a conserved gene of unknown function that is strongly induced in response to many stress conditions and repressed in drug resistant yeast strains. Gene expression patterns of YER067W and its paralog YIL057C suggest an involvement in energy metabolism. We show that yeast lacking YER067W display altered levels of reserve carbohydrates and a growth deficiency in media that requires aerobic metabolism. Impaired mitochondrial function and overall reduction of ergosterol content in the YER067W deleted strain explained the observed 2- and 4-fold increase in resistance to the drugs fluconazole and amphotericin B, respectively. Cell fractionation and immunofluorescence microscopy revealed that Yer067w is associated with cellular membranes despite the absence of a transmembrane domain in the protein. Finally, the 1.7 A resolution crystal structure of Yer067w shows an alpha-beta fold with low similarity to known structures and a putative functional site.YER067W's involvement with aerobic energetic metabolism suggests the assignment of the gene name RGI1, standing for respiratory growth induced 1. Altogether, the results shed light on a previously uncharacterized protein family and provide basis for further studies of its apparent role in energy metabolism control and drug resistance.
Structural and functional study of YER067W, a new protein involved in yeast metabolism control and drug resistance.,Domitrovic T, Kozlov G, Freire JC, Masuda CA, da Silva Almeida M, Montero-Lomeli M, Atella GC, Matta-Camacho E, Gehring K, Kurtenbach E PLoS One. 2010 Jun 17;5(6):e11163. PMID:20567505[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Domitrovic T, Kozlov G, Freire JC, Masuda CA, da Silva Almeida M, Montero-Lomeli M, Atella GC, Matta-Camacho E, Gehring K, Kurtenbach E. Structural and functional study of YER067W, a new protein involved in yeast metabolism control and drug resistance. PLoS One. 2010 Jun 17;5(6):e11163. PMID:20567505 doi:10.1371/journal.pone.0011163
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