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| | <StructureSection load='4jj3' size='340' side='right'caption='[[4jj3]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='4jj3' size='340' side='right'caption='[[4jj3]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4jj3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Magmm Magmm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JJ3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4JJ3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4jj3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Magnetococcus_marinus_MC-1 Magnetococcus marinus MC-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JJ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JJ3 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4jj0|4jj0]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4jj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jj3 OCA], [http://pdbe.org/4jj3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4jj3 RCSB], [http://www.ebi.ac.uk/pdbsum/4jj3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4jj3 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jj3 OCA], [https://pdbe.org/4jj3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jj3 RCSB], [https://www.ebi.ac.uk/pdbsum/4jj3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jj3 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0L9W2_MAGMM A0L9W2_MAGMM] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Magmm]] | + | [[Category: Magnetococcus marinus MC-1]] |
| - | [[Category: Arnoux, P]] | + | [[Category: Arnoux P]] |
| - | [[Category: Pignol, D]] | + | [[Category: Pignol D]] |
| - | [[Category: Siponen, M]] | + | [[Category: Siponen M]] |
| - | [[Category: Cytochrome]]
| + | |
| - | [[Category: Electron transport]]
| + | |
| - | [[Category: Magnetochrome]]
| + | |
| - | [[Category: Pdz]]
| + | |
| Structural highlights
Function
A0L9W2_MAGMM
Publication Abstract from PubMed
Magnetotactic bacteria align along the Earth's magnetic field using an organelle called the magnetosome, a biomineralized magnetite (Fe(II)Fe(III)2O4) or greigite (Fe(II)Fe(III)2S4) crystal embedded in a lipid vesicle. Although the need for both iron(II) and iron(III) is clear, little is known about the biological mechanisms controlling their ratio. Here we present the structure of the magnetosome-associated protein MamP and find that it is built on a unique arrangement of a self-plugged PDZ domain fused to two magnetochrome domains, defining a new class of c-type cytochrome exclusively found in magnetotactic bacteria. Mutational analysis, enzyme kinetics, co-crystallization with iron(II) and an in vitro MamP-assisted magnetite production assay establish MamP as an iron oxidase that contributes to the formation of iron(III) ferrihydrite eventually required for magnetite crystal growth in vivo. These results demonstrate the molecular mechanisms of iron management taking place inside the magnetosome and highlight the role of magnetochrome in iron biomineralization.
Structural insight into magnetochrome-mediated magnetite biomineralization.,Siponen MI, Legrand P, Widdrat M, Jones SR, Zhang WJ, Chang MC, Faivre D, Arnoux P, Pignol D Nature. 2013 Oct 31;502(7473):681-4. doi: 10.1038/nature12573. Epub 2013 Oct 6. PMID:24097349[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Siponen MI, Legrand P, Widdrat M, Jones SR, Zhang WJ, Chang MC, Faivre D, Arnoux P, Pignol D. Structural insight into magnetochrome-mediated magnetite biomineralization. Nature. 2013 Oct 31;502(7473):681-4. doi: 10.1038/nature12573. Epub 2013 Oct 6. PMID:24097349 doi:http://dx.doi.org/10.1038/nature12573
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