6m4f
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the E496A mutant of HsBglA== | |
| + | <StructureSection load='6m4f' size='340' side='right'caption='[[6m4f]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6m4f]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Hamamotoa_singularis Hamamotoa singularis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M4F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6M4F FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m4f OCA], [https://pdbe.org/6m4f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m4f RCSB], [https://www.ebi.ac.uk/pdbsum/6m4f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m4f ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q564N5_9BASI Q564N5_9BASI] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A GH1 beta-glucosidase from the fungus Hamamotoa singularis (HsBglA) has high transgalactosylation activity and efficiently converts lactose to galactooligosaccharides. Consequently, HsBglA is among the most widely used enzymes for industrial galactooligosaccharide production. Here, we present the first crystal structures of HsBglA with and without 4'-galactosyllactose, a tri-galactooligosaccharide, at 3.0 and 2.1 A resolutions, respectively. These structures reveal details of the structural elements that define the catalytic activity and substrate binding of HsBglA, and provide a possible interpretation for its high catalytic potency for transgalactosylation reaction. | ||
| - | + | Crystal structure of a GH1 beta-glucosidase from Hamamotoa singularis.,Uehara R, Iwamoto R, Aoki S, Yoshizawa T, Takano K, Matsumura H, Tanaka SI Protein Sci. 2020 Jul 26. doi: 10.1002/pro.3916. PMID:32713015<ref>PMID:32713015</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6m4f" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Hamamotoa singularis]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Aoki S]] | ||
| + | [[Category: Iwamoto R]] | ||
| + | [[Category: Matsumura H]] | ||
| + | [[Category: Takano K]] | ||
| + | [[Category: Tanaka S-i]] | ||
| + | [[Category: Uehara R]] | ||
| + | [[Category: Yoshizawa T]] | ||
Current revision
Crystal structure of the E496A mutant of HsBglA
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