7bxz
From Proteopedia
(Difference between revisions)
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==Crystal structure of the aminoglycoside 6'-N-acetyltransferase from Enterococcus faecium== | ==Crystal structure of the aminoglycoside 6'-N-acetyltransferase from Enterococcus faecium== | ||
| - | <StructureSection load='7bxz' size='340' side='right'caption='[[7bxz]]' scene=''> | + | <StructureSection load='7bxz' size='340' side='right'caption='[[7bxz]], [[Resolution|resolution]] 2.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BXZ OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[7bxz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BXZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BXZ FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.502Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bxz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bxz OCA], [https://pdbe.org/7bxz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bxz RCSB], [https://www.ebi.ac.uk/pdbsum/7bxz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bxz ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q47764_ENTFC Q47764_ENTFC] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Aminoglycoside acetyltransferases (AACs) catalyze the transfer of an acetyl group between acetyl-CoA and an aminoglycoside, producing CoA and an acetylated aminoglycoside. AAC(6')-Ii enzymes target the amino group linked to the 6' C atom in an aminoglycoside. Several structures of the AAC(6')-Ii from Enterococcus faecium [Ef-AAC(6')-Ii] have been reported to date. However, the detailed mechanism of its enzymatic function remains elusive. In this study, the crystal structure of Ef-AAC(6')-Ii was determined in a novel substrate-free form. Based on structural analysis, it is proposed that Ef-AAC(6')-Ii sequentially undergoes conformational selection and induced fit for substrate binding. These results therefore provide a novel viewpoint on the mechanism of action of Ef-AAC(6')-Ii. | ||
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| + | Structural analysis of a novel substrate-free form of the aminoglycoside 6'-N-acetyltransferase from Enterococcus faecium.,Jang H, Kwon S, Jeong CS, Lee CW, Hwang J, Jung KH, Lee JH, Park HH Acta Crystallogr F Struct Biol Commun. 2020 Aug 1;76(Pt 8):364-371. doi:, 10.1107/S2053230X20009735. Epub 2020 Jul 28. PMID:32744248<ref>PMID:32744248</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7bxz" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Enterococcus faecium]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Kwon S]] | [[Category: Kwon S]] | ||
[[Category: Park HH]] | [[Category: Park HH]] | ||
Current revision
Crystal structure of the aminoglycoside 6'-N-acetyltransferase from Enterococcus faecium
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