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3bzg

From Proteopedia

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Current revision

UVDE pH4.4

Structural highlights

3bzg is a 1 chain structure with sequence from Thermus thermophilus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.91Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q746K1_THET2

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

UV damage endonuclease is a DNA repair enzyme that can both recognize damage such as UV lesions and introduce a nick directly 5' to them. Recently, the crystal structure of the enzyme from Thermus thermophilus was solved. In the electron density map of this structure, unexplained density near the active site was observed at the tip of Lys229. Based on this finding, it was proposed that Lys229 is post-translationally modified. In this article, we give evidence that this modification is a carboxyl group. By combining activity assays and X-ray crystallography on several point mutants, we show that the carboxyl group assists in metal binding required for catalysis by donating negative charge to the metal-coordinating residue His231. Moreover, functional and structural analysis of the K229R mutant reveals that if His231 shifts away, an increased activity results on both damaged and undamaged DNA. Taken together, the results show that T. thermophilus ultraviolet damage endonuclease is carboxylated and the modified lysine is required for proper catalysis and preventing increased incision of undamaged DNA.

Involvement of a carboxylated lysine in UV damage endonuclease.,Meulenbroek EM, Paspaleva K, Thomassen EA, Abrahams JP, Goosen N, Pannu NS Protein Sci. 2009 Mar;18(3):549-58. PMID:19241382^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Meulenbroek EM, Paspaleva K, Thomassen EA, Abrahams JP, Goosen N, Pannu NS. Involvement of a carboxylated lysine in UV damage endonuclease. Protein Sci. 2009 Mar;18(3):549-58. PMID:19241382 doi:10.1002/pro.54

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3bzg"

@@ Line 3: / Line 3: @@
 <StructureSection load='3bzg' size='340' side='right'caption='[[3bzg]], [[Resolution|resolution]] 1.91&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3bzg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"flavobacterium_thermophilum"_yoshida_and_oshima_1971 "flavobacterium thermophilum" yoshida and oshima 1971]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3BZG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3bzg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BZG FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3bzj|3bzj]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.91&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3bzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzg OCA], [http://pdbe.org/3bzg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bzg RCSB], [http://www.ebi.ac.uk/pdbsum/3bzg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bzg ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzg OCA], [https://pdbe.org/3bzg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bzg RCSB], [https://www.ebi.ac.uk/pdbsum/3bzg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bzg ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q746K1_THET2 Q746K1_THET2]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 33: / Line 35: @@
 __TOC__
 </StructureSection>
-[[Category: Flavobacterium thermophilum yoshida and oshima 1971]]
 [[Category: Large Structures]]
-[[Category: Abrahams, J P]]
+[[Category: Thermus thermophilus]]
-[[Category: Goosen, N]]
+[[Category: Abrahams JP]]
-[[Category: Meulenbroek, E M]]
+[[Category: Goosen N]]
-[[Category: Pannu, N S]]
+[[Category: Meulenbroek EM]]
-[[Category: Paspaleva, K]]
+[[Category: Pannu NS]]
-[[Category: Thomassen, E A.J]]
+[[Category: Paspaleva K]]
-[[Category: Dna repair]]
+[[Category: Thomassen EAJ]]
-[[Category: Endonuclease]]
-[[Category: Hydrolase]]
-[[Category: Tim barrel]]
-[[Category: Uvde]]

3bzg

From Proteopedia

Current revision

UVDE pH4.4

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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