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| | <StructureSection load='3c0s' size='340' side='right'caption='[[3c0s]], [[Resolution|resolution]] 1.80Å' scene=''> | | <StructureSection load='3c0s' size='340' side='right'caption='[[3c0s]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3c0s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"flavobacterium_thermophilum"_yoshida_and_oshima_1971 "flavobacterium thermophilum" yoshida and oshima 1971]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C0S OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3C0S FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3c0s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C0S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C0S FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3bzg|3bzg]], [[3bzj|3bzj]], [[3c0l|3c0l]], [[3c0q|3c0q]]</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c0s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c0s OCA], [https://pdbe.org/3c0s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c0s RCSB], [https://www.ebi.ac.uk/pdbsum/3c0s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c0s ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3c0s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c0s OCA], [http://pdbe.org/3c0s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3c0s RCSB], [http://www.ebi.ac.uk/pdbsum/3c0s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3c0s ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q746K1_THET2 Q746K1_THET2] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Flavobacterium thermophilum yoshida and oshima 1971]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Abrahams, J P]] | + | [[Category: Thermus thermophilus]] |
| - | [[Category: Goosen, N]] | + | [[Category: Abrahams JP]] |
| - | [[Category: Meulenbroek, E M]] | + | [[Category: Goosen N]] |
| - | [[Category: Pannu, N S]] | + | [[Category: Meulenbroek EM]] |
| - | [[Category: Paspaleva, K]] | + | [[Category: Pannu NS]] |
| - | [[Category: Thomassen, E A.J]] | + | [[Category: Paspaleva K]] |
| - | [[Category: Dna repair]]
| + | [[Category: Thomassen EAJ]] |
| - | [[Category: Endonuclease]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Plasmid]]
| + | |
| - | [[Category: Tim barrel]]
| + | |
| - | [[Category: Uvde]]
| + | |
| Structural highlights
Function
Q746K1_THET2
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
UV damage endonuclease is a DNA repair enzyme that can both recognize damage such as UV lesions and introduce a nick directly 5' to them. Recently, the crystal structure of the enzyme from Thermus thermophilus was solved. In the electron density map of this structure, unexplained density near the active site was observed at the tip of Lys229. Based on this finding, it was proposed that Lys229 is post-translationally modified. In this article, we give evidence that this modification is a carboxyl group. By combining activity assays and X-ray crystallography on several point mutants, we show that the carboxyl group assists in metal binding required for catalysis by donating negative charge to the metal-coordinating residue His231. Moreover, functional and structural analysis of the K229R mutant reveals that if His231 shifts away, an increased activity results on both damaged and undamaged DNA. Taken together, the results show that T. thermophilus ultraviolet damage endonuclease is carboxylated and the modified lysine is required for proper catalysis and preventing increased incision of undamaged DNA.
Involvement of a carboxylated lysine in UV damage endonuclease.,Meulenbroek EM, Paspaleva K, Thomassen EA, Abrahams JP, Goosen N, Pannu NS Protein Sci. 2009 Mar;18(3):549-58. PMID:19241382[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Meulenbroek EM, Paspaleva K, Thomassen EA, Abrahams JP, Goosen N, Pannu NS. Involvement of a carboxylated lysine in UV damage endonuclease. Protein Sci. 2009 Mar;18(3):549-58. PMID:19241382 doi:10.1002/pro.54
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