3ca9

<table><tr><td colspan='2'>[[3ca9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlorella_virus_il-3a Chlorella virus il-3a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CA9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3CA9 FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DUD:DEOXYURIDINE-5-DIPHOSPHATE'>DUD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3c2t|3c2t]], [[3c3i|3c3i]]</td></tr>

<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] </span></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3ca9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ca9 OCA], [http://pdbe.org/3ca9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ca9 RCSB], [http://www.ebi.ac.uk/pdbsum/3ca9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ca9 ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

The 141-amino-acid deoxyuridine triphosphatase (dUTPase) from the algal Chlorella virus IL-3A and its Glu81Ser/Thr84Arg-mutant derivative Mu-22 were crystallized using the hanging-drop vapor-diffusion method at 298 K with polyethylene glycol as the precipitant. An apo IL-3A dUTPase with an amino-terminal T7 epitope tag and a carboxy-terminal histidine tag yielded cubic P2(1)3 crystals with unit-cell parameter a = 106.65 A. In the presence of dUDP, the enzyme produced thin stacked orthorhombic P222 crystals with unit-cell parameters a = 81.0, b = 96.2, c = 132.8 A. T7-histidine-tagged Mu-22 dUTPase formed thin stacked rectangular crystals. Amino-terminal histidine-tagged dUTPases did not crystallize but formed aggregates. Glycyl-seryl-tagged dUTPases yielded cubic P2(1)3 IL-3A crystals with unit-cell parameter a = 105.68 A and hexagonal P6(3) Mu-22 crystals with unit-cell parameters a = 132.07, c = 53.45 A, gamma = 120 degrees . Owing to the Thr84Arg mutation, Mu-22 dUTPase had different monomer-to-monomer interactions to those of IL-3A dUTPase.

Crystallization and crystal-packing studies of Chlorella virus deoxyuridine triphosphatase.,Homma K, Moriyama H Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Oct 1;65(Pt, 10):1030-4. Epub 2009 Sep 25. PMID:19851015<ref>PMID:19851015</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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== References ==

<references/>

[[Category: Chlorella virus il-3a]]

[[Category: DUTP diphosphatase]]

[[Category: Etten, L V.J]]

[[Category: Homma, K]]

[[Category: Moriyama, H]]

[[Category: Yamanishi, M]]

[[Category: Zhang, Y]]

[[Category: Hydrolase]]