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5lte

From Proteopedia

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Current revision

Crystal structure of the alpha subunit of heme dependent oxidative N-demethylase (HODM)

Structural highlights

5lte is a 1 chain structure with sequence from Pseudomonas mendocina ymp. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.65Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A4XXY9_PSEMY

Publication Abstract from PubMed

The universal Per-ARNT-Sim (PAS) domain functions as a signal transduction module involved in sensing diverse stimuli such as small molecules, light, redox state and gases. The highly evolvable PAS scaffold can bind a broad range of ligands, including haem, flavins and metal ions. However, although these ligands can support catalytic activity, to our knowledge no enzymatic PAS domain has been found. Here we report characterization of the first PAS enzyme: a haem-dependent oxidative N-demethylase. Unrelated to other amine oxidases, this enzyme contains haem, flavin mononucleotide, 2Fe-2S and tetrahydrofolic acid cofactors, and specifically catalyses the NADPH-dependent oxidation of dimethylamine. The structure of the alpha subunit reveals that it is a haem-binding PAS domain, similar in structure to PAS gas sensors. The dimethylamine substrate forms part of a highly polarized oxygen-binding site, and directly assists oxygen activation by acting as both an electron and proton donor. Our data reveal that the ubiquitous PAS domain can make the transition from sensor to enzyme, suggesting that the PAS scaffold can support the development of artificial enzymes.

An oxidative N-demethylase reveals PAS transition from ubiquitous sensor to enzyme.,Ortmayer M, Lafite P, Menon BR, Tralau T, Fisher K, Denkhaus L, Scrutton NS, Rigby SE, Munro AW, Hay S, Leys D Nature. 2016 Nov 24;539(7630):593-597. doi: 10.1038/nature20159. Epub 2016 Nov, 16. PMID:27851736^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ortmayer M, Lafite P, Menon BR, Tralau T, Fisher K, Denkhaus L, Scrutton NS, Rigby SE, Munro AW, Hay S, Leys D. An oxidative N-demethylase reveals PAS transition from ubiquitous sensor to enzyme. Nature. 2016 Nov 24;539(7630):593-597. doi: 10.1038/nature20159. Epub 2016 Nov, 16. PMID:27851736 doi:http://dx.doi.org/10.1038/nature20159

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5lte"

Categories: Large Structures | Pseudomonas mendocina ymp | Leys D | Ortmayer M

@@ Line 3: / Line 3: @@
 <StructureSection load='5lte' size='340' side='right'caption='[[5lte]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5lte]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Psemy Psemy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LTE OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LTE FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5lte]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_mendocina_ymp Pseudomonas mendocina ymp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LTE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LTE FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ETX:2-ETHOXYETHANOL'>ETX</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Pmen_3455 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=399739 PSEMY])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ETX:2-ETHOXYETHANOL'>ETX</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5lte FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lte OCA], [http://pdbe.org/5lte PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lte RCSB], [http://www.ebi.ac.uk/pdbsum/5lte PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lte ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lte FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lte OCA], [https://pdbe.org/5lte PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lte RCSB], [https://www.ebi.ac.uk/pdbsum/5lte PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lte ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A4XXY9_PSEMY A4XXY9_PSEMY]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 24: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Psemy]]
+[[Category: Pseudomonas mendocina ymp]]
-[[Category: Leys, D]]
+[[Category: Leys D]]
-[[Category: Ortmayer, M]]
+[[Category: Ortmayer M]]
-[[Category: Amine oxidase]]
-[[Category: Heme binding]]
-[[Category: Oxidoreductase]]
-[[Category: Pas domain]]

5lte

From Proteopedia

Current revision

Crystal structure of the alpha subunit of heme dependent oxidative N-demethylase (HODM)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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