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| - | ==Crystal structure of core domain of a Pili-like protein== | + | ==Crystal structure of the core domain of Amuc_1100 from Akkermansia muciniphila== |
| | <StructureSection load='6laf' size='340' side='right'caption='[[6laf]], [[Resolution|resolution]] 3.00Å' scene=''> | | <StructureSection load='6laf' size='340' side='right'caption='[[6laf]], [[Resolution|resolution]] 3.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6laf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Akkm8 Akkm8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LAF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LAF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6laf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Akkermansia_muciniphila_ATCC_BAA-835 Akkermansia muciniphila ATCC BAA-835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LAF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LAF FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.001Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6lad|6lad]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Amuc_1100 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=349741 AKKM8])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6laf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6laf OCA], [https://pdbe.org/6laf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6laf RCSB], [https://www.ebi.ac.uk/pdbsum/6laf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6laf ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6laf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6laf OCA], [http://pdbe.org/6laf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6laf RCSB], [http://www.ebi.ac.uk/pdbsum/6laf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6laf ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/B2UR41_AKKM8 B2UR41_AKKM8] |
| - | Akkermansia muciniphila is a beneficial microorganism colonized in the human gut that can reverse many intestinal metabolic-related diseases. Amuc_1100 is an outer-membrane protein of A. muciniphila. Oral administration of Amuc_1100 can reduce fat mass development, insulin resistance, and dyslipidemia in mice and activated the toll-like receptor 2 (TLR2) to regulate the immune response of the host, but the molecular mechanism remains unclear. Here we report the crystal structure of the extramembranous domain of Amuc_1100, which consists of a four-stranded antiparallel beta-sheet and four alpha-helices. Two C-terminal helices and the four-stranded antiparallel beta-sheet formed two "alphabetabeta" motifs and constituted the core domain, which shared a similar fold with type IV pili and type II Secretion system protein. Although the full-length of the extramembranous domain of Amuc_1100 existed as a monomer in solution, they formed trimer in the crystal. Elimination of the N-terminal coiled-coil helix alpha1 led to dimerization of Amuc_1100 both in solution and in crystal, indicating that the oligomeric state of Amuc_1100 was variable and could be influenced by alpha1. In addition, we identified that Amuc_1100 could directly bind human TLR2 (hTRL2) in vitro, suggesting that Amuc_1100 may serve as a new ligand for hTLR2. Dimerization of Amuc_1100 improved its hTLR2-binding affinity, suggesting that the alpha1-truncated Amuc_1100 could be a beneficial candidate for the development of A. muciniphila related drugs.
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| - | The variable oligomeric state of Amuc_1100 from Akkermansia muciniphila.,Wang J, Xiang R, Wang R, Zhang B, Gong W, Zhang J, Zhang M, Wang M J Struct Biol. 2020 Jul 28:107593. doi: 10.1016/j.jsb.2020.107593. PMID:32736072<ref>PMID:32736072</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
| + | |
| - | <div class="pdbe-citations 6laf" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Akkm8]] | + | [[Category: Akkermansia muciniphila ATCC BAA-835]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Wang, J]] | + | [[Category: Wang J]] |
| - | [[Category: Wang, M]] | + | [[Category: Wang M]] |
| - | [[Category: Xiang, R]] | + | [[Category: Xiang R]] |
| - | [[Category: Zhang, M]] | + | [[Category: Zhang M]] |
| - | [[Category: Akkermansia muciniphila]]
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| - | [[Category: Amuc_1100]]
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| - | [[Category: Membrane protein]]
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| - | [[Category: Outer-membrane protein]]
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| - | [[Category: Toll-like receptor 2]]
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| - | [[Category: Type ii secretion system]]
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