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| | <StructureSection load='3dih' size='340' side='right'caption='[[3dih]], [[Resolution|resolution]] 2.60Å' scene=''> | | <StructureSection load='3dih' size='340' side='right'caption='[[3dih]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3dih]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vipera_ammodytes_ammodytes Vipera ammodytes ammodytes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DIH OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3DIH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3dih]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vipera_ammodytes_ammodytes Vipera ammodytes ammodytes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DIH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DIH FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3dih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dih OCA], [http://pdbe.org/3dih PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3dih RCSB], [http://www.ebi.ac.uk/pdbsum/3dih PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3dih ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dih OCA], [https://pdbe.org/3dih PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dih RCSB], [https://www.ebi.ac.uk/pdbsum/3dih PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dih ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PA2HL_VIPAA PA2HL_VIPAA]] Snake venom phospholipase A2 homolog that is very active in inducing myonecrosis in vivo and shows a potent calcium-independent membrane-damaging activity in vitro, most probably by binding and incorporating in the membrane. Also acts as a presynaptic neurotoxin.<ref>PMID:10653156</ref> <ref>PMID:11005629</ref> <ref>PMID:1765075</ref> <ref>PMID:17927217</ref> <ref>PMID:7662700</ref> <ref>PMID:8835336</ref> <ref>PMID:8973554</ref> <ref>PMID:9514950</ref> | + | [https://www.uniprot.org/uniprot/PA2HL_VIPAA PA2HL_VIPAA] Snake venom phospholipase A2 homolog that is very active in inducing myonecrosis in vivo and shows a potent calcium-independent membrane-damaging activity in vitro, most probably by binding and incorporating in the membrane. Also acts as a presynaptic neurotoxin.<ref>PMID:10653156</ref> <ref>PMID:11005629</ref> <ref>PMID:1765075</ref> <ref>PMID:17927217</ref> <ref>PMID:7662700</ref> <ref>PMID:8835336</ref> <ref>PMID:8973554</ref> <ref>PMID:9514950</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | <jmolCheckbox> | | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/di/3dih_consurf.spt"</scriptWhenChecked> | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/di/3dih_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> | | </jmolCheckbox> |
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Vipera ammodytes ammodytes]] | | [[Category: Vipera ammodytes ammodytes]] |
| - | [[Category: Guncar, G]] | + | [[Category: Guncar G]] |
| - | [[Category: Krizaj, I]] | + | [[Category: Krizaj I]] |
| - | [[Category: Turk, D]] | + | [[Category: Turk D]] |
| - | [[Category: Myotoxin]]
| + | |
| - | [[Category: Phospholipase a2 fold]]
| + | |
| - | [[Category: Secreted]]
| + | |
| - | [[Category: Toxin]]
| + | |
| Structural highlights
Function
PA2HL_VIPAA Snake venom phospholipase A2 homolog that is very active in inducing myonecrosis in vivo and shows a potent calcium-independent membrane-damaging activity in vitro, most probably by binding and incorporating in the membrane. Also acts as a presynaptic neurotoxin.[1] [2] [3] [4] [5] [6] [7] [8]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
See Also
References
- ↑ Horvat-Znidarsic I, Suput D, Gubensek F. The effect of ammodytin L on frog neuromuscular junction. Pflugers Arch. 2000;439(3 Suppl):R102-3. PMID:10653156
- ↑ Frangez R, Krizaj I, Gubensek F, Suput D. Effects of ammodytin L on miniature and endplate potentials in neuromuscular junction of frog m. cutaneus pectoris. Pflugers Arch. 2000;440(5 Suppl):R101-2. PMID:11005629
- ↑ Krizaj I, Bieber AL, Ritonja A, Gubensek F. The primary structure of ammodytin L, a myotoxic phospholipase A2 homologue from Vipera ammodytes venom. Eur J Biochem. 1991 Dec 18;202(3):1165-8. PMID:1765075
- ↑ Petan T, Krizaj I, Pungercar J. Restoration of enzymatic activity in a Ser-49 phospholipase A2 homologue decreases its Ca(2+)-independent membrane-damaging activity and increases its toxicity. Biochemistry. 2007 Nov 6;46(44):12795-809. Epub 2007 Oct 10. PMID:17927217 doi:http://dx.doi.org/10.1021/bi701304e
- ↑ Incerpi S, de Vito P, Luly P, Rufini S. Effect of ammodytin L from Vipera ammodytes on L-6 cells from rat skeletal muscle. Biochim Biophys Acta. 1995 Aug 31;1268(2):137-42. PMID:7662700
- ↑ Bernardini S, Cannata S, Filoni S, Luly P, Rufini S. Effect of ammodytin L from the venom of Vipera ammodytes on Xenopus laevis differentiated muscle fibres and regenerating limbs. Toxicon. 1996 Jan;34(1):81-90. PMID:8835336
- ↑ Rufini S, Cesaroni MP, Balestro N, Luly P. Proliferative effect of ammodytin L from the venom of Vipera ammodytes on 208F rat fibroblasts in culture. Biochem J. 1996 Dec 1;320 ( Pt 2):467-72. PMID:8973554
- ↑ Pungercar J, Vucemilo N, Faure G, Bon C, Verheij HM, Gubensek F, Krizaj I. Ammodytin L, an inactive phospholipase A2 homologue with myotoxicity in mice, binds to the presynaptic acceptor of the beta-neurotoxic ammodytoxin C in Torpedo: an indication for a phospholipase A2 activity-independent mechanism of action of beta-neurotoxins in fish? Biochem Biophys Res Commun. 1998 Mar 17;244(2):514-8. PMID:9514950
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