This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4cas

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Serial femtosecond crystallography structure of a photosynthetic reaction center

Structural highlights

4cas is a 4 chain structure with sequence from Blastochloris viridis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.5Å
Ligands:	, , , , , , , , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CYCR_BLAVI The reaction center of purple bacteria contains a tightly bound cytochrome molecule which re-reduces the photo oxidized primary electron donor.^[1]

Publication Abstract from PubMed

Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 A resolution and determine its serial femtosecond crystallography structure to 3.5 A resolution. Although every microcrystal is exposed to a dose of 33 MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.

Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography.,Johansson LC, Arnlund D, Katona G, White TA, Barty A, Deponte DP, Shoeman RL, Wickstrand C, Sharma A, Williams GJ, Aquila A, Bogan MJ, Caleman C, Davidsson J, Doak RB, Frank M, Fromme R, Galli L, Grotjohann I, Hunter MS, Kassemeyer S, Kirian RA, Kupitz C, Liang M, Lomb L, Malmerberg E, Martin AV, Messerschmidt M, Nass K, Redecke L, Seibert MM, Sjohamn J, Steinbrener J, Stellato F, Wang D, Wahlgren WY, Weierstall U, Westenhoff S, Zatsepin NA, Boutet S, Spence JC, Schlichting I, Chapman HN, Fromme P, Neutze R Nat Commun. 2013 Dec 19;4:2911. doi: 10.1038/ncomms3911. PMID:24352554^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chen IP, Mathis P, Koepke J, Michel H. Uphill electron transfer in the tetraheme cytochrome subunit of the Rhodopseudomonas viridis photosynthetic reaction center: evidence from site-directed mutagenesis. Biochemistry. 2000 Apr 4;39(13):3592-602. PMID:10736158
↑ Johansson LC, Arnlund D, Katona G, White TA, Barty A, Deponte DP, Shoeman RL, Wickstrand C, Sharma A, Williams GJ, Aquila A, Bogan MJ, Caleman C, Davidsson J, Doak RB, Frank M, Fromme R, Galli L, Grotjohann I, Hunter MS, Kassemeyer S, Kirian RA, Kupitz C, Liang M, Lomb L, Malmerberg E, Martin AV, Messerschmidt M, Nass K, Redecke L, Seibert MM, Sjohamn J, Steinbrener J, Stellato F, Wang D, Wahlgren WY, Weierstall U, Westenhoff S, Zatsepin NA, Boutet S, Spence JC, Schlichting I, Chapman HN, Fromme P, Neutze R. Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography. Nat Commun. 2013 Dec 19;4:2911. doi: 10.1038/ncomms3911. PMID:24352554 doi:http://dx.doi.org/10.1038/ncomms3911

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4cas"

@@ Line 3: / Line 3: @@
 <StructureSection load='4cas' size='340' side='right'caption='[[4cas]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4cas]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Blastochloris_viridis Blastochloris viridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CAS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4CAS FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4cas]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Blastochloris_viridis Blastochloris viridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CAS FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPB:BACTERIOPHEOPHYTIN+B'>BPB</scene>, <scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MPG:[(Z)-OCTADEC-9-ENYL]+(2R)-2,3-BIS(OXIDANYL)PROPANOATE'>MPG</scene>, <scene name='pdbligand=MQ7:MENAQUINONE-7'>MQ7</scene>, <scene name='pdbligand=NS5:15-CIS-1,2-DIHYDRONEUROSPORENE'>NS5</scene>, <scene name='pdbligand=OTP:(2E,6E,10E,14E,18E,22E,26E)-3,7,11,15,19,23,27,31-OCTAMETHYLDOTRIACONTA-2,6,10,14,18,22,26,30-OCTAENYL+TRIHYDROGEN+DIPHOSPHATE'>OTP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPB:BACTERIOPHEOPHYTIN+B'>BPB</scene>, <scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MPG:[(Z)-OCTADEC-9-ENYL]+(2R)-2,3-BIS(OXIDANYL)PROPANOATE'>MPG</scene>, <scene name='pdbligand=MQ7:MENAQUINONE-7'>MQ7</scene>, <scene name='pdbligand=NS5:15-CIS-1,2-DIHYDRONEUROSPORENE'>NS5</scene>, <scene name='pdbligand=OTP:(2E,6E,10E,14E,18E,22E,26E)-3,7,11,15,19,23,27,31-OCTAMETHYLDOTRIACONTA-2,6,10,14,18,22,26,30-OCTAENYL+TRIHYDROGEN+DIPHOSPHATE'>OTP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4cas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cas OCA], [http://pdbe.org/4cas PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cas RCSB], [http://www.ebi.ac.uk/pdbsum/4cas PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cas ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cas OCA], [https://pdbe.org/4cas PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cas RCSB], [https://www.ebi.ac.uk/pdbsum/4cas PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cas ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/RCEH_RHOVI RCEH_RHOVI]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis. [[http://www.uniprot.org/uniprot/CYCR_RHOVI CYCR_RHOVI]] The reaction center of purple bacteria contains a tightly bound cytochrome molecule which re-reduces the photo oxidized primary electron donor. [[http://www.uniprot.org/uniprot/RCEM_RHOVI RCEM_RHOVI]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis. [[http://www.uniprot.org/uniprot/RCEL_RHOVI RCEL_RHOVI]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
+[https://www.uniprot.org/uniprot/CYCR_BLAVI CYCR_BLAVI] The reaction center of purple bacteria contains a tightly bound cytochrome molecule which re-reduces the photo oxidized primary electron donor.<ref>PMID:10736158</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 25: @@
 [[Category: Blastochloris viridis]]
 [[Category: Large Structures]]
-[[Category: Aquila, A]]
+[[Category: Aquila A]]
-[[Category: Arnlund, D]]
+[[Category: Arnlund D]]
-[[Category: Barty, A]]
+[[Category: Barty A]]
-[[Category: Bogan, M J]]
+[[Category: Bogan MJ]]
-[[Category: Boutet, S]]
+[[Category: Boutet S]]
-[[Category: Caleman, C]]
+[[Category: Caleman C]]
-[[Category: Chapman, H N]]
+[[Category: Chapman HN]]
-[[Category: Davidsson, J]]
+[[Category: Davidsson J]]
-[[Category: DePonte, D P]]
+[[Category: DePonte DP]]
-[[Category: Doak, R B]]
+[[Category: Doak RB]]
-[[Category: Frank, M]]
+[[Category: Frank M]]
-[[Category: Fromme, P]]
+[[Category: Fromme P]]
-[[Category: Fromme, R]]
+[[Category: Fromme R]]
-[[Category: Galli, L]]
+[[Category: Galli L]]
-[[Category: Grotjohann, I]]
+[[Category: Grotjohann I]]
-[[Category: Hunter, M S]]
+[[Category: Hunter MS]]
-[[Category: Johansson, L C]]
+[[Category: Johansson LC]]
-[[Category: Kassemeyer, S]]
+[[Category: Kassemeyer S]]
-[[Category: Katona, G]]
+[[Category: Katona G]]
-[[Category: Kirian, R A]]
+[[Category: Kirian RA]]
-[[Category: Kupitz, C]]
+[[Category: Kupitz C]]
-[[Category: Liang, M]]
+[[Category: Liang M]]
-[[Category: Lomb, L]]
+[[Category: Lomb L]]
-[[Category: Malmerberg, E]]
+[[Category: Malmerberg E]]
-[[Category: Martin, A V]]
+[[Category: Martin AV]]
-[[Category: Messerschmidt, M]]
+[[Category: Messerschmidt M]]
-[[Category: Nass, K]]
+[[Category: Nass K]]
-[[Category: Neutze, R]]
+[[Category: Neutze R]]
-[[Category: Redecke, L]]
+[[Category: Redecke L]]
-[[Category: Schlichting, I]]
+[[Category: Schlichting I]]
-[[Category: Seibert, M M]]
+[[Category: Seibert MM]]
-[[Category: Sharma, A]]
+[[Category: Sharma A]]
-[[Category: Shoeman, R L]]
+[[Category: Shoeman RL]]
-[[Category: Sjohamn, J]]
+[[Category: Sjohamn J]]
-[[Category: Spence, J C.H]]
+[[Category: Spence JCH]]
-[[Category: Steinbrener, J]]
+[[Category: Steinbrener J]]
-[[Category: Stellato, F]]
+[[Category: Stellato F]]
-[[Category: Wahlgren, W Y]]
+[[Category: Wahlgren WY]]
-[[Category: Wang, D]]
+[[Category: Wang D]]
-[[Category: Weierstall, U]]
+[[Category: Weierstall U]]
-[[Category: Westenhoff, S]]
+[[Category: Westenhoff S]]
-[[Category: White, T A]]
+[[Category: White TA]]
-[[Category: Wickstrand, C]]
+[[Category: Wickstrand C]]
-[[Category: Williams, G J]]
+[[Category: Williams GJ]]
-[[Category: Zatsepin, N A]]
+[[Category: Zatsepin NA]]
-[[Category: Cell membrane]]
-[[Category: Chromophore]]
-[[Category: Electron transport]]
-[[Category: Lipidic-sponge phase]]
-[[Category: Metal- binding]]
-[[Category: Photosynthesis]]
-[[Category: Reaction center]]
-[[Category: Transmembrane]]

4cas

From Proteopedia

Current revision

Serial femtosecond crystallography structure of a photosynthetic reaction center

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox