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5nny

From Proteopedia

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Current revision

Crystal structure of the phosphatase domain from the Legionella effector WipB

Structural highlights

5nny is a 2 chain structure with sequence from Legionella pneumophila. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.7Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q5GA15_LEGPN

Publication Abstract from PubMed

Legionella pneumophila infects human alveolar macrophages and is responsible for Legionnaire's disease, a severe form of pneumonia. L. pneumophila encodes more than 300 putative effectors, which are translocated into the host cell via the Dot/Icm type IV secretion system. These effectors highjack the host's cellular processes to allow bacterial intracellular growth and replication. Here we adopted a multidisciplinary approach to investigate WipB, a Dot/Icm effector of unknown function. The crystal structure of the N-terminal domain at 1.7 A resolution comprising residues 25 to 344 revealed that WipB harbours a Ser/Thr phosphatase domain related to the eukaryotic phospho-protein phosphatase (PPP) family. The C-terminal domain (residues 365-524) is sufficient to pilot the effector to acidified LAMP1-positive lysosomal compartments, where WipB interacts with the v-ATPase and the associated LAMTOR1 phosphoprotein, key components of the lysosomal nutrient sensing (LYNUS) apparatus that controls the mammalian target of rapamycin (mTORC1) kinase complex at the lysosomal surface. We propose that WipB is a lysosome-targeted phosphatase that modulates cellular nutrient sensing and the control of energy metabolism during Legionella infection.

The Legionella effector WipB is a translocated Ser/Thr phosphatase that targets the host lysosomal nutrient sensing machinery.,Prevost MS, Pinotsis N, Dumoux M, Hayward RD, Waksman G Sci Rep. 2017 Aug 25;7(1):9450. doi: 10.1038/s41598-017-10249-6. PMID:28842705^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Prevost MS, Pinotsis N, Dumoux M, Hayward RD, Waksman G. The Legionella effector WipB is a translocated Ser/Thr phosphatase that targets the host lysosomal nutrient sensing machinery. Sci Rep. 2017 Aug 25;7(1):9450. doi: 10.1038/s41598-017-10249-6. PMID:28842705 doi:http://dx.doi.org/10.1038/s41598-017-10249-6

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5nny"

Categories: Large Structures | Legionella pneumophila | Pinotsis N | Prevost MS | Waksman G

@@ Line 3: / Line 3: @@
 <StructureSection load='5nny' size='340' side='right'caption='[[5nny]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5nny]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NNY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5NNY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5nny]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NNY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NNY FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5nny FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nny OCA], [http://pdbe.org/5nny PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nny RCSB], [http://www.ebi.ac.uk/pdbsum/5nny PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nny ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nny FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nny OCA], [https://pdbe.org/5nny PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nny RCSB], [https://www.ebi.ac.uk/pdbsum/5nny PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nny ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q5GA15_LEGPN Q5GA15_LEGPN]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 23: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Pinotsis, N]]
+[[Category: Legionella pneumophila]]
-[[Category: Prevost, M S]]
+[[Category: Pinotsis N]]
-[[Category: Waksman, G]]
+[[Category: Prevost MS]]
-[[Category: Hydrolase]]
+[[Category: Waksman G]]
-[[Category: Legionella effector]]
-[[Category: Phosphoesterase fold]]
-[[Category: Serine/threonine phosphatase]]

5nny

From Proteopedia

Current revision

Crystal structure of the phosphatase domain from the Legionella effector WipB

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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