7cls
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Solution structure of an intramolecular G-quadruplex containing a duplex bulge== | |
| + | <StructureSection load='7cls' size='340' side='right'caption='[[7cls]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7cls]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CLS FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cls OCA], [https://pdbe.org/7cls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cls RCSB], [https://www.ebi.ac.uk/pdbsum/7cls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cls ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Beyond the consensus definition of G-quadruplex-forming motifs with tracts of continuous guanines, G-quadruplexes harboring bulges in the G-tetrad core are prevalent in the human genome. Here, we study the incorporation of a duplex hairpin within a bulge of a G-quadruplex. The NMR solution structure of a G-quadruplex containing a duplex bulge was resolved, revealing the structural details of the junction between the duplex bulge and the G-quadruplex. Unexpectedly, instead of an orthogonal connection the duplex stem was observed to stack below the G-quadruplex forming a unique quadruplex-duplex junction. Breaking up of the immediate base pair step at the junction, coupled with a narrowing of the duplex groove within the context of the bulge, led to a progressive transition between the quadruplex and duplex segments. This study revealed that a duplex bulge can be formed at various positions of a G-quadruplex scaffold. In contrast to a non-structured bulge, the stability of a G-quadruplex slightly increases with an increase in the duplex bulge size. A G-quadruplex structure containing a duplex bulge of up to 33 nt in size was shown to form, which was much larger than the previously reported 7-nt bulge. With G-quadruplexes containing duplex bulges representing new structural motifs with potential biological significance, our findings would broaden the definition of potential G-quadruplex-forming sequences. | ||
| - | + | Duplex formation in a G-quadruplex bulge.,Ngoc Nguyen TQ, Lim KW, Phan AT Nucleic Acids Res. 2020 Sep 22. pii: 5909921. doi: 10.1093/nar/gkaa738. PMID:32960213<ref>PMID:32960213</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 7cls" style="background-color:#fffaf0;"></div> |
| - | [[Category: Lim | + | == References == |
| - | [[Category: Phan | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Lim KW]] | ||
| + | [[Category: Nguyen TQN]] | ||
| + | [[Category: Phan AT]] | ||
Current revision
Solution structure of an intramolecular G-quadruplex containing a duplex bulge
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