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| | <StructureSection load='6kog' size='340' side='right'caption='[[6kog]], [[Resolution|resolution]] 1.68Å' scene=''> | | <StructureSection load='6kog' size='340' side='right'caption='[[6kog]], [[Resolution|resolution]] 1.68Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6kog]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mago7 Mago7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KOG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KOG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6kog]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyricularia_oryzae_70-15 Pyricularia oryzae 70-15]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KOG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KOG FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.68Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TAS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=242507 MAGO7])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kog OCA], [http://pdbe.org/6kog PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kog RCSB], [http://www.ebi.ac.uk/pdbsum/6kog PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kog ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kog OCA], [https://pdbe.org/6kog PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kog RCSB], [https://www.ebi.ac.uk/pdbsum/6kog PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kog ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TAS1_MAGO7 TAS1_MAGO7]] Hybrid PKS-NRPS synthetase that mediates the biosynthesis of the toxin tenuazonic acid (TeA), an inhibitor of protein biosynthesis on ribosomes by suppressing the release of new protein (PubMed:26503170). TAS1 alone is sufficient for TeA synthesis (PubMed:26503170). The NRPS part performs the condensation of isoleucine with acetoacetyl-CoA and the final cyclization step is conducted by the KS domain (PubMed:26503170).<ref>PMID:26503170</ref> | + | [https://www.uniprot.org/uniprot/TAS1_MAGO7 TAS1_MAGO7] Hybrid PKS-NRPS synthetase that mediates the biosynthesis of the toxin tenuazonic acid (TeA), an inhibitor of protein biosynthesis on ribosomes by suppressing the release of new protein (PubMed:26503170). TAS1 alone is sufficient for TeA synthesis (PubMed:26503170). The NRPS part performs the condensation of isoleucine with acetoacetyl-CoA and the final cyclization step is conducted by the KS domain (PubMed:26503170).<ref>PMID:26503170</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Mago7]] | + | [[Category: Pyricularia oryzae 70-15]] |
| - | [[Category: Hino, T]] | + | [[Category: Hino T]] |
| - | [[Category: Motoyama, T]] | + | [[Category: Motoyama T]] |
| - | [[Category: Nagano, S]] | + | [[Category: Nagano S]] |
| - | [[Category: Nishimoto, K]] | + | [[Category: Nishimoto K]] |
| - | [[Category: Osada, H]] | + | [[Category: Osada H]] |
| - | [[Category: Yun, C S]] | + | [[Category: Yun CS]] |
| - | [[Category: Biosynthetic protein]]
| + | |
| - | [[Category: Cyclization]]
| + | |
| - | [[Category: Ketosynthase domain]]
| + | |
| - | [[Category: Nrps-pks hybrid enzyme]]
| + | |
| - | [[Category: Tetramic acid ring formation]]
| + | |
| - | [[Category: Toxin]]
| + | |
| Structural highlights
Function
TAS1_MAGO7 Hybrid PKS-NRPS synthetase that mediates the biosynthesis of the toxin tenuazonic acid (TeA), an inhibitor of protein biosynthesis on ribosomes by suppressing the release of new protein (PubMed:26503170). TAS1 alone is sufficient for TeA synthesis (PubMed:26503170). The NRPS part performs the condensation of isoleucine with acetoacetyl-CoA and the final cyclization step is conducted by the KS domain (PubMed:26503170).[1]
Publication Abstract from PubMed
Many microbial secondary metabolites are produced by multienzyme complexes comprising non-ribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs). The ketosynthase (KS) domains of polyketide synthase normally catalyze the decarboxylative Claisen condensation of acyl and malonyl blocks to extend the polyketide chain. However, the terminal KS domain in tenuazonic acid synthetase 1 (TAS1) from the fungus Pyricularia oryzae conducts substrate cyclization. Here, we report on the unique features of the KS domain in TAS1. We observed that this domain is monomeric, not dimeric as is typical for KSs. Analysis of a 1.68 A-resolution crystal structure suggests that the substrate cyclization is triggered via proton abstraction from the active methylene moiety in the substrate by a catalytic His-322 residue. Additionally, we show that TAS1 KS promiscuously accepts amino acyl substrates and that this promiscuity can be increased by a single amino acid substitution in the substrate-binding pocket of the enzyme. These findings provide insight into a KS domain that accepts the amino acid-containing substrate in an NRPS-PKS hybrid enzyme and provide hints to the substrate cyclization mechanism performed by the KS domain in the biosynthesis of the mycotoxin tenuazonic acid.
Unique features of the ketosynthase domain in a non-ribosomal peptide synthetase-polyketide synthase hybrid enzyme, tenuazonic acid synthetase 1.,Yun CS, Nishimoto K, Motoyama T, Shimizu T, Hino T, Dohmae N, Nagano S, Osada H J Biol Chem. 2020 Jun 21. pii: RA120.013105. doi: 10.1074/jbc.RA120.013105. PMID:32565425[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yun CS, Motoyama T, Osada H. Biosynthesis of the mycotoxin tenuazonic acid by a fungal NRPS-PKS hybrid enzyme. Nat Commun. 2015 Oct 27;6:8758. doi: 10.1038/ncomms9758. PMID:26503170 doi:http://dx.doi.org/10.1038/ncomms9758
- ↑ Yun CS, Nishimoto K, Motoyama T, Shimizu T, Hino T, Dohmae N, Nagano S, Osada H. Unique features of the ketosynthase domain in a non-ribosomal peptide synthetase-polyketide synthase hybrid enzyme, tenuazonic acid synthetase 1. J Biol Chem. 2020 Jun 21. pii: RA120.013105. doi: 10.1074/jbc.RA120.013105. PMID:32565425 doi:http://dx.doi.org/10.1074/jbc.RA120.013105
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