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| | <StructureSection load='6p7l' size='340' side='right'caption='[[6p7l]], [[Resolution|resolution]] 1.80Å' scene=''> | | <StructureSection load='6p7l' size='340' side='right'caption='[[6p7l]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6p7l]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._cm020 Streptomyces sp. cm020]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P7L OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6P7L FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6p7l]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._CM020 Streptomyces sp. CM020]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P7L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6P7L FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aln2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=569580 Streptomyces sp. CM020])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6p7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p7l OCA], [http://pdbe.org/6p7l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p7l RCSB], [http://www.ebi.ac.uk/pdbsum/6p7l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p7l ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6p7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p7l OCA], [https://pdbe.org/6p7l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6p7l RCSB], [https://www.ebi.ac.uk/pdbsum/6p7l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6p7l ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/B6SEE8_9ACTN B6SEE8_9ACTN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Streptomyces sp. cm020]] | + | [[Category: Streptomyces sp. CM020]] |
| - | [[Category: Silvaggi, N R]] | + | [[Category: Silvaggi NR]] |
| - | [[Category: Vuksanovic, N]] | + | [[Category: Vuksanovic N]] |
| - | [[Category: Alnumycin]]
| + | |
| - | [[Category: Antibiotic]]
| + | |
| Structural highlights
Function
B6SEE8_9ACTN
Publication Abstract from PubMed
Proteins belonging to the NTF2-like superfamily are present in the biosynthetic pathways of numerous polyketide natural products, such as anthracyclins and benzoisochromanequinones. Some have been found to be bona fide polyketide cyclases, but many of them have roles that are currently unknown. Here, the X-ray crystal structures of three NTF2-like proteins of unknown function are reported: those of ActVI-ORFA from Streptomyces coelicolor A3(2) and its homologs Caci_6494, a protein from an uncharacterized biosynthetic cluster in Catenulispora acidiphila, and Aln2 from Streptomyces sp. CM020, a protein in the biosynthetic pathway of alnumycin. The presence of a solvent-accessible cavity and the conservation of the His/Asp dyad that is characteristic of many polyketide cyclases suggest a potential enzymatic role for these enzymes in polyketide biosynthesis.
Structural characterization of three noncanonical NTF2-like superfamily proteins: implications for polyketide biosynthesis.,Vuksanovic N, Zhu X, Serrano DA, Siitonen V, Metsa-Ketela M, Melancon CE 3rd, Silvaggi NR Acta Crystallogr F Struct Biol Commun. 2020 Aug 1;76(Pt 8):372-383. doi:, 10.1107/S2053230X20009814. Epub 2020 Jul 29. PMID:32744249[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Vuksanovic N, Zhu X, Serrano DA, Siitonen V, Metsa-Ketela M, Melancon CE 3rd, Silvaggi NR. Structural characterization of three noncanonical NTF2-like superfamily proteins: implications for polyketide biosynthesis. Acta Crystallogr F Struct Biol Commun. 2020 Aug 1;76(Pt 8):372-383. doi:, 10.1107/S2053230X20009814. Epub 2020 Jul 29. PMID:32744249 doi:http://dx.doi.org/10.1107/S2053230X20009814
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