7crk
From Proteopedia
(Difference between revisions)
| (2 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==2ps Structure of Chloride ion pumping rhodopsin (ClR) with NTQ motif== | |
| + | <StructureSection load='7crk' size='340' side='right'caption='[[7crk]], [[Resolution|resolution]] 1.85Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CRK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CRK FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7crk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7crk OCA], [https://pdbe.org/7crk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7crk RCSB], [https://www.ebi.ac.uk/pdbsum/7crk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7crk ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Chloride ion-pumping rhodopsin (ClR) in some marine bacteria utilizes light energy to actively transport Cl(-) into cells. How the ClR initiates the transport is elusive. Here, we show the dynamics of ion transport observed with time-resolved serial femtosecond (fs) crystallography using the Linac Coherent Light Source. X-ray pulses captured structural changes in ClR upon flash illumination with a 550 nm fs-pumping laser. High-resolution structures for five time points (dark to 100 ps after flashing) reveal complex and coordinated dynamics comprising retinal isomerization, water molecule rearrangement, and conformational changes of various residues. Combining data from time-resolved spectroscopy experiments and molecular dynamics simulations, this study reveals that the chloride ion close to the Schiff base undergoes a dissociation-diffusion process upon light-triggered retinal isomerization. | ||
| - | + | Early-stage dynamics of chloride ion-pumping rhodopsin revealed by a femtosecond X-ray laser.,Yun JH, Li X, Yue J, Park JH, Jin Z, Li C, Hu H, Shi Y, Pandey S, Carbajo S, Boutet S, Hunter MS, Liang M, Sierra RG, Lane TJ, Zhou L, Weierstall U, Zatsepin NA, Ohki M, Tame JRH, Park SY, Spence JCH, Zhang W, Schmidt M, Lee W, Liu H Proc Natl Acad Sci U S A. 2021 Mar 30;118(13). pii: 2020486118. doi:, 10.1073/pnas.2020486118. PMID:33753488<ref>PMID:33753488</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 7crk" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[Rhodopsin 3D structures|Rhodopsin 3D structures]] |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Lee WT]] | ||
| + | [[Category: Liu H]] | ||
| + | [[Category: Schmidt M]] | ||
| + | [[Category: Yun JH]] | ||
Current revision
2ps Structure of Chloride ion pumping rhodopsin (ClR) with NTQ motif
| |||||||||||
Categories: Large Structures | Lee WT | Liu H | Schmidt M | Yun JH
