This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


6m76

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:48, 27 March 2024) (edit) (undo)
 
Line 3: Line 3:
<StructureSection load='6m76' size='340' side='right'caption='[[6m76]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
<StructureSection load='6m76' size='340' side='right'caption='[[6m76]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[6m76]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis_tx4248 Enterococcus faecalis tx4248]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M76 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6M76 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[6m76]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis_ATCC_10100 Enterococcus faecalis ATCC 10100]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M76 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6M76 FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6m76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m76 OCA], [http://pdbe.org/6m76 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m76 RCSB], [http://www.ebi.ac.uk/pdbsum/6m76 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m76 ProSAT]</span></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m76 OCA], [https://pdbe.org/6m76 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m76 RCSB], [https://www.ebi.ac.uk/pdbsum/6m76 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m76 ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/A0A125W693_ENTFL A0A125W693_ENTFL]
-
Glycoside hydrolases catalyze the hydrolysis of glycosidic linkages in carbohydrates. The glycoside hydrolase family 31 (GH31) contains alpha-glucosidase, alpha-xylosidase, alpha-galactosidase, and alpha-transglycosylase. Recent work has expanded the diversity of substrate specificity of GH31 enzymes, and alpha-N-acetylgalactosaminidases (alphaGalNAcases) belonging to GH31 have been identified in human gut bacteria. Here, we determined the first crystal structure of a truncated form of GH31 alphaGalNAcase from the human gut bacterium Enterococcus faecalis. The enzyme has a similar fold to other reported GH31 enzymes and an additional fibronectin type 3-like domain. Additionally, the structure in complex with N-acetylgalactosamine reveals that conformations of the active site residues, including its catalytic nucleophile, change to recognize the ligand. Our structural analysis provides insight into the substrate recognition and catalytic mechanism of GH31 alphaGalNAcases.
+
-
 
+
-
Crystal structure of the Enterococcus faecalis alpha-N-acetylgalactosaminidase, a member of the glycoside hydrolase family 31.,Miyazaki T, Park EY FEBS Lett. 2020 May 4. doi: 10.1002/1873-3468.13804. PMID:32367553<ref>PMID:32367553</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 6m76" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Enterococcus faecalis tx4248]]
+
[[Category: Enterococcus faecalis ATCC 10100]]
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Miyazaki, T]]
+
[[Category: Miyazaki T]]
-
[[Category: Fibronectin-like]]
+
-
[[Category: Glycoside hydrolase]]
+
-
[[Category: Hydrolase]]
+
-
[[Category: Mucin]]
+

Current revision

GH31 alpha-N-acetylgalactosaminidase from Enterococcus faecalis

PDB ID 6m76

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6m76"
Personal tools