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| | ==NMR solution structure of ligand-free OAA== | | ==NMR solution structure of ligand-free OAA== |
| - | <StructureSection load='2mwh' size='340' side='right'caption='[[2mwh]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2mwh' size='340' side='right'caption='[[2mwh]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2mwh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"planktothrix_agardhii"_emend._suda_et_al._2002 "planktothrix agardhii" emend. suda et al. 2002]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MWH OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2MWH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2mwh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Planktothrix_agardhii Planktothrix agardhii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MWH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MWH FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2mwh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mwh OCA], [http://pdbe.org/2mwh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mwh RCSB], [http://www.ebi.ac.uk/pdbsum/2mwh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mwh ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mwh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mwh OCA], [https://pdbe.org/2mwh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mwh RCSB], [https://www.ebi.ac.uk/pdbsum/2mwh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mwh ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/LEC1_PLAAG LEC1_PLAAG] Lectin specific for high mannose N-glycans, recognizes the branched moiety of these glycans. Does not recognize other types of N-glycans or monosaccharides. Agglutinates trypsin-treated rabbit erythrocytes. Does not require divalent cations for activity. Inhibits HIV replication in MT4 cells with an EC(50) of 45 nM. Binds to the HIV envelope glycoprotein gp120.<ref>PMID:10817903</ref> <ref>PMID:17314091</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Planktothrix agardhii emend. suda et al. 2002]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ban, D]] | + | [[Category: Planktothrix agardhii]] |
| - | [[Category: Carneiro, M G]] | + | [[Category: Ban D]] |
| - | [[Category: Griesinger, C]] | + | [[Category: Carneiro MG]] |
| - | [[Category: Gronenborn, A M]] | + | [[Category: Griesinger C]] |
| - | [[Category: Koharudin, L M]] | + | [[Category: Gronenborn AM]] |
| - | [[Category: Lee, D]] | + | [[Category: Koharudin LM]] |
| - | [[Category: Mazur, A]] | + | [[Category: Lee D]] |
| - | [[Category: Sabo, T]] | + | [[Category: Mazur A]] |
| - | [[Category: Trigo-Mourino, P]] | + | [[Category: Sabo T]] |
| - | [[Category: Protein binding]]
| + | [[Category: Trigo-Mourino P]] |
| - | [[Category: Sugar binding protein-antiviral protein complex]]
| + | |
| Structural highlights
Function
LEC1_PLAAG Lectin specific for high mannose N-glycans, recognizes the branched moiety of these glycans. Does not recognize other types of N-glycans or monosaccharides. Agglutinates trypsin-treated rabbit erythrocytes. Does not require divalent cations for activity. Inhibits HIV replication in MT4 cells with an EC(50) of 45 nM. Binds to the HIV envelope glycoprotein gp120.[1] [2]
Publication Abstract from PubMed
Lectins from different sources have been shown to interfere with HIV infection by binding to the sugars of viral-envelope glycoproteins. Three-dimensional atomic structures of a number of HIV-inactivating lectins have been determined, both as free proteins and in glycan-bound forms. However, details on the mechanism of recognition and binding to sugars are elusive. Herein we focus on the anti-HIV lectin OAA from Oscillatoria agardhii: We show that in the absence of sugars in solution, both the sugar-free and sugar-bound protein conformations that were observed in the X-ray crystal structures exist as conformational substates. Our results suggest that glycan recognition occurs by conformational selection within the ground state; this model differs from the popular "excited-state" model. Our findings provide further insight into molecular recognition of the major receptor on the HIV virus by OAA. These details can potentially be used for the optimization and/or development of preventive anti-HIV therapeutics.
Sampling of Glycan-Bound Conformers by the Anti-HIV Lectin Oscillatoria agardhii agglutinin in the Absence of Sugar.,Carneiro MG, Koharudin LM, Ban D, Sabo TM, Trigo-Mourino P, Mazur A, Griesinger C, Gronenborn AM, Lee D Angew Chem Int Ed Engl. 2015 Apr 14. doi: 10.1002/anie.201500213. PMID:25873445[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sato Y, Murakami M, Miyazawa K, Hori K. Purification and characterization of a novel lectin from a freshwater cyanobacterium, Oscillatoria agardhii. Comp Biochem Physiol B Biochem Mol Biol. 2000 Feb;125(2):169-77. PMID:10817903 doi:10.1016/s0305-0491(99)00164-9
- ↑ Sato Y, Okuyama S, Hori K. Primary structure and carbohydrate binding specificity of a potent anti-HIV lectin isolated from the filamentous cyanobacterium Oscillatoria agardhii. J Biol Chem. 2007 Apr 13;282(15):11021-9. Epub 2007 Feb 21. PMID:17314091 doi:10.1074/jbc.M701252200
- ↑ Carneiro MG, Koharudin LM, Ban D, Sabo TM, Trigo-Mourino P, Mazur A, Griesinger C, Gronenborn AM, Lee D. Sampling of Glycan-Bound Conformers by the Anti-HIV Lectin Oscillatoria agardhii agglutinin in the Absence of Sugar. Angew Chem Int Ed Engl. 2015 Apr 14. doi: 10.1002/anie.201500213. PMID:25873445 doi:http://dx.doi.org/10.1002/anie.201500213
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