3hzk

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of S73-2 antibody in complex with antigen Kdo(2.4)Kdo

Structural highlights

3hzk is a 2 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.15Å
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The structures of antigen-binding fragments from two related monoclonal antibodies have been determined to high resolution in the presence of several carbohydrate antigens raised against chlamydial lipopolysaccharide. With the exception of CDR H3, antibodies S54-10 and S73-2 are both derived from the same set of germline gene segments as the previously reported structures S25-2 and S45-18. Despite this similarity, the antibodies differ in specificity and the mechanism by which they recognize their cognate antigen. S54-10 uses an unrelated CDR H3 to recognize its antigen in a fashion analogous to S45-18; however, S73-2 recognizes the same antigen as S45-18 and S54-10 in a wholly unrelated manner. Together, these antibody-antigen structures provide snapshots into how the immune system uses the same set of inherited germline gene segments to generate multiple possible specificities that allow for differential recognition of epitopes and how unrelated CDR H3 sequences can result in convergent binding of clinically relevant bacterial antigens.

Antibodies Raised Against Chlamydial Lipopolysaccharide Antigens Reveal Convergence in Germline Gene Usage and Differential Epitope Recognition.,Brooks CL, Muller-Loennies S, Borisova SN, Brade L, Kosma P, Hirama T, Mackenzie CR, Brade H, Evans SV Biochemistry. 2009 Dec 31. PMID:20000757^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Brooks CL, Muller-Loennies S, Borisova SN, Brade L, Kosma P, Hirama T, Mackenzie CR, Brade H, Evans SV. Antibodies Raised Against Chlamydial Lipopolysaccharide Antigens Reveal Convergence in Germline Gene Usage and Differential Epitope Recognition. Biochemistry. 2009 Dec 31. PMID:20000757 doi:10.1021/bi9011308

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3hzk"

@@ Line 3: / Line 3: @@
 <StructureSection load='3hzk' size='340' side='right'caption='[[3hzk]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3hzk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3HZK FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3hzk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HZK FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=KDA:(3-DEOXY-D-MANNO-OCT-2-ULOSONIC+ACID)-2-O-ALLYL'>KDA</scene>, <scene name='pdbligand=KDO:3-DEOXY-D-MANNO-OCT-2-ULOSONIC+ACID'>KDO</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hzm|3hzm]], [[3hzv|3hzv]], [[3hzy|3hzy]], [[3i02|3i02]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KDA:(3-DEOXY-D-MANNO-OCT-2-ULOSONIC+ACID)-2-O-ALLYL'>KDA</scene>, <scene name='pdbligand=KDO:3-DEOXY-D-MANNO-OCT-2-ULOSONIC+ACID'>KDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3hzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hzk OCA], [http://pdbe.org/3hzk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hzk RCSB], [http://www.ebi.ac.uk/pdbsum/3hzk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hzk ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hzk OCA], [https://pdbe.org/3hzk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hzk RCSB], [https://www.ebi.ac.uk/pdbsum/3hzk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hzk ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 13: / Line 13: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hz/3hzk_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 37: / Line 37: @@
 [[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Borisova, S N]]
+[[Category: Borisova SN]]
-[[Category: Brade, H]]
+[[Category: Brade H]]
-[[Category: Brade, L]]
+[[Category: Brade L]]
-[[Category: Brooks, C L]]
+[[Category: Brooks CL]]
-[[Category: Evans, S V]]
+[[Category: Evans SV]]
-[[Category: Hirama, T]]
+[[Category: Hirama T]]
-[[Category: Kosma, P]]
+[[Category: Kosma P]]
-[[Category: MacKenzie, C R]]
+[[Category: MacKenzie CR]]
-[[Category: Muller-Loennies, S]]
+[[Category: Muller-Loennies S]]
-[[Category: Antibody]]
-[[Category: Carbohydrate]]
-[[Category: Fab]]
-[[Category: Igg]]
-[[Category: Immune system]]

3hzk

From Proteopedia

Current revision

Crystal structure of S73-2 antibody in complex with antigen Kdo(2.4)Kdo

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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