1ar1

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="1ar1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ar1, resolution 2.7&Aring;" /> '''STRUCTURE AT 2.7 ANG...)
Current revision (15:27, 13 March 2024) (edit) (undo)
 
(23 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1ar1.gif|left|200px]]<br />
 
-
<applet load="1ar1" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1ar1, resolution 2.7&Aring;" />
 
-
'''STRUCTURE AT 2.7 ANGSTROM RESOLUTION OF THE PARACOCCUS DENITRIFICANS TWO-SUBUNIT CYTOCHROME C OXIDASE COMPLEXED WITH AN ANTIBODY FV FRAGMENT'''<br />
 
-
==Overview==
+
==Structure at 2.7 Angstrom Resolution of the Paracoccus Denitrificans two-subunit Cytochrome C Oxidase Complexed with an Antibody Fv Fragment==
-
The aa3 type cytochrome c oxidase consisting of the core subunits I and II, only was isolated from the soil bacterium Paracoccus denitrificans and, crystallized as complex with a monoclonal antibody Fv fragment. Crystals, could be grown in the presence of a number of different nonionic, detergents. However, only undecyl-beta-D-maltoside and, cyclohexyl-hexyl-beta-D-maltoside yielded well-ordered crystals suitable, for high resolution x-ray crystallographic studies. The crystals belong to, space group P212121 and diffract x-rays to at least 2.5 A (1 A = 0.1 nm), resolution using synchrotron radiation. The structure was determined to a, resolution of 2.7 A using molecular replacement and refined to a, crystallographic R-factor of 20.5% (Rfree = 25.9%). The refined model, includes subunits I ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9380672 (full description)]]
+
<StructureSection load='1ar1' size='340' side='right'caption='[[1ar1]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1ar1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AR1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AR1 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=HEA:HEME-A'>HEA</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ar1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ar1 OCA], [https://pdbe.org/1ar1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ar1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ar1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ar1 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/COX1B_PARDE COX1B_PARDE] Subunit I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B). This cytochrome c oxidase shows proton pump activity across the membrane in addition to the electron transfer.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ar/1ar1_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ar1 ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1AR1 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]] and [[http://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans]] with CU, MG, CA, HEA and LDA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.9.3.1 1.9.3.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AR1 OCA]].
+
*[[Cytochrome c oxidase 3D structures|Cytochrome c oxidase 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Structure at 2.7 A resolution of the Paracoccus denitrificans two-subunit cytochrome c oxidase complexed with an antibody FV fragment., Ostermeier C, Harrenga A, Ermler U, Michel H, Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10547-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9380672 9380672]
+
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Paracoccus denitrificans]]
[[Category: Paracoccus denitrificans]]
-
[[Category: Protein complex]]
+
[[Category: Ermler U]]
-
[[Category: Ermler, U.]]
+
[[Category: Harrenga A]]
-
[[Category: Harrenga, A.]]
+
[[Category: Michel H]]
-
[[Category: Michel, H.]]
+
[[Category: Ostermeier C]]
-
[[Category: Ostermeier, C.]]
+
-
[[Category: CA]]
+
-
[[Category: CU]]
+
-
[[Category: HEA]]
+
-
[[Category: LDA]]
+
-
[[Category: MG]]
+
-
[[Category: antibody complex]]
+
-
[[Category: complex (oxidoreductase/antibody)]]
+
-
[[Category: cytochrome oxidase]]
+
-
[[Category: electron transport]]
+
-
[[Category: transmembrane]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 17:55:34 2007''
+

Current revision

Structure at 2.7 Angstrom Resolution of the Paracoccus Denitrificans two-subunit Cytochrome C Oxidase Complexed with an Antibody Fv Fragment

PDB ID 1ar1

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ar1"
Personal tools