|
|
| Line 3: |
Line 3: |
| | <StructureSection load='6y7q' size='340' side='right'caption='[[6y7q]], [[Resolution|resolution]] 1.39Å' scene=''> | | <StructureSection load='6y7q' size='340' side='right'caption='[[6y7q]], [[Resolution|resolution]] 1.39Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6y7q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y7Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Y7Q FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6y7q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y7Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Y7Q FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.39Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5va1|5va1]], [[5va2|5va2]], [[5va3|5va3]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KCNH7, ERG3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6y7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y7q OCA], [https://pdbe.org/6y7q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6y7q RCSB], [https://www.ebi.ac.uk/pdbsum/6y7q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6y7q ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6y7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y7q OCA], [http://pdbe.org/6y7q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6y7q RCSB], [http://www.ebi.ac.uk/pdbsum/6y7q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6y7q ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/KCNH7_HUMAN KCNH7_HUMAN]] Pore-forming (alpha) subunit of voltage-gated potassium channel. Channel properties may be modulated by cAMP and subunit assembly. | + | [https://www.uniprot.org/uniprot/KCNH7_HUMAN KCNH7_HUMAN] Pore-forming (alpha) subunit of voltage-gated potassium channel. Channel properties may be modulated by cAMP and subunit assembly. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 24: |
Line 23: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Cresser-Brown, J]] | + | [[Category: Cresser-Brown J]] |
| - | [[Category: Moody, P]] | + | [[Category: Moody P]] |
| - | [[Category: Raven, E]] | + | [[Category: Raven E]] |
| - | [[Category: Heme binding]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Pa]]
| + | |
| - | [[Category: Potassium channel]]
| + | |
| - | [[Category: Rossmann fold]]
| + | |
| Structural highlights
Function
KCNH7_HUMAN Pore-forming (alpha) subunit of voltage-gated potassium channel. Channel properties may be modulated by cAMP and subunit assembly.
Publication Abstract from PubMed
The ether-a-go-go (EAG) family of voltage gated K+ channels are important regulators of neuronal and cardiac action potential firing (excitability) and have major roles in human diseases such as epilepsy, schizophrenia, cancer and sudden cardiac death. A defining feature of EAG (Kv10-12) channels is a highly conserved domain on the amino-terminus, known as the eag-domain, consisting of a PAS domain capped by a short sequence containing an amphipathic helix (Cap-domain). The PAS and Cap domains are both vital for the normal function of EAG channels. Using heme-affinity pull-down assays and proteomics of lysates from primary cortical neurons, we identified that an EAG channel, hERG3 (Kv11.3), binds to heme. In whole cell electrophysiology experiments, we identified that heme inhibits hERG3 channel activity. In addition, we expressed the Cap and PAS domain of hERG3 in E.coli and, using spectroscopy and kinetics, identified the PAS domain as the location for heme binding. The results identify heme as a regulator of hERG3 channel activity. These observations are discussed in the context of the emerging role for heme as a regulator of ion channel activity in cells.
Discovery of a heme-binding domain in a neuronal voltage-gated potassium channel.,Burton MJ, Cresser-Brown J, Thomas M, Portolano N, Basran J, Freeman SL, Kwon H, Bottrill AR, Llansola-Portoles MJ, Pascal AA, Jukes-Jones R, Chernova T, Schmid R, Davies NW, Storey NM, Dorlet P, Moody PCE, Mitcheson JS, Raven EL J Biol Chem. 2020 Jul 28. pii: RA120.014150. doi: 10.1074/jbc.RA120.014150. PMID:32723862[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Burton MJ, Cresser-Brown J, Thomas M, Portolano N, Basran J, Freeman SL, Kwon H, Bottrill AR, Llansola-Portoles MJ, Pascal AA, Jukes-Jones R, Chernova T, Schmid R, Davies NW, Storey NM, Dorlet P, Moody PCE, Mitcheson JS, Raven EL. Discovery of a heme-binding domain in a neuronal voltage-gated potassium channel. J Biol Chem. 2020 Jul 28. pii: RA120.014150. doi: 10.1074/jbc.RA120.014150. PMID:32723862 doi:http://dx.doi.org/10.1074/jbc.RA120.014150
|
