6z1z

<table><tr><td colspan='2'>[[6z1z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z1Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Z1Z FirstGlance]. <br>

</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6rlr|6rlr]], [[6z1v|6z1v]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6z1z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z1z OCA], [http://pdbe.org/6z1z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6z1z RCSB], [http://www.ebi.ac.uk/pdbsum/6z1z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6z1z ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Tetraspanins are eukaryotic membrane proteins that contribute to a variety of signaling processes by organizing partner-receptor molecules in the plasma membrane. How tetraspanins bind and cluster partner receptors into tetraspanin-enriched microdomains is unknown. Here, we present crystal structures of the large extracellular loop of CD9 bound to nanobodies 4C8 and 4E8 and, the cryo-EM structure of 4C8-bound CD9 in complex with its partner EWI-F. CD9-EWI-F displays a tetrameric arrangement with two central EWI-F molecules, dimerized through their ectodomains, and two CD9 molecules, one bound to each EWI-F transmembrane helix through CD9-helices h3 and h4. In the crystal structures, nanobodies 4C8 and 4E8 bind CD9 at loops C and D, which is in agreement with the 4C8 conformation in the CD9-EWI-F complex. The complex varies from nearly twofold symmetric (with the two CD9 copies nearly anti-parallel) to ca. 50 degrees bent arrangements. This flexible arrangement of CD9-EWI-F with potential CD9 homo-dimerization at either end provides a "concatenation model" for forming short linear or circular assemblies, which may explain the occurrence of tetraspanin-enriched microdomains.

Implications for tetraspanin-enriched microdomain assembly based on structures of CD9 with EWI-F.,Oosterheert W, Xenaki KT, Neviani V, Pos W, Doulkeridou S, Manshande J, Pearce NM, Kroon-Batenburg LM, Lutz M, van Bergen En Henegouwen PM, Gros P Life Sci Alliance. 2020 Sep 21;3(11). pii: 3/11/e202000883. doi:, 10.26508/lsa.202000883. Print 2020 Nov. PMID:32958604<ref>PMID:32958604</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 6z1z" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Camelus glama]]

[[Category: Gros, P]]

[[Category: Kroon-Batenburg, L M.J]]

[[Category: Lutz, M]]

[[Category: Neviani, N]]

[[Category: Oosterheert, W]]

[[Category: Pearce, N M]]

[[Category: Nanobody]]