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5v5f

From Proteopedia

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Current revision (14:31, 6 March 2024) (edit) (undo)
 
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<StructureSection load='5v5f' size='340' side='right'caption='[[5v5f]], [[Resolution|resolution]] 2.94&Aring;' scene=''>
<StructureSection load='5v5f' size='340' side='right'caption='[[5v5f]], [[Resolution|resolution]] 2.94&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5v5f]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V5F OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5V5F FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5v5f]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V5F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5V5F FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5v5f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v5f OCA], [http://pdbe.org/5v5f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5v5f RCSB], [http://www.ebi.ac.uk/pdbsum/5v5f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5v5f ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.945&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5v5f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v5f OCA], [https://pdbe.org/5v5f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5v5f RCSB], [https://www.ebi.ac.uk/pdbsum/5v5f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5v5f ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/RICE1_ARATH RICE1_ARATH] 3'-to-5' exoribonuclease (RNase) specifically targeting single-stranded RNAs (PubMed:28463111). Triggers miRNA accumulation in RNA-induced silencing complex (RISC), composed of miRNAs and AGO proteins, by degrading uridylated cleavage fragments (PubMed:28463111). Required during plant growth and development (PubMed:28463111).<ref>PMID:28463111</ref>
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RNA-induced silencing complex (RISC) is composed of miRNAs and AGO proteins. AGOs use miRNAs as guides to slice target mRNAs to produce truncated 5' and 3' RNA fragments. The 5' cleaved RNA fragments are marked with uridylation for degradation. Here, we identified novel cofactors of Arabidopsis AGOs, named RICE1 and RICE2. RICE proteins specifically degraded single-strand (ss) RNAs in vitro; but neither miRNAs nor miRNA*s in vivo. RICE1 exhibited a DnaQ-like exonuclease fold and formed a homohexamer with the active sites located at the interfaces between RICE1 subunits. Notably, ectopic expression of catalytically-inactive RICE1 not only significantly reduced miRNA levels; but also increased 5' cleavage RISC fragments with extended uridine tails. We conclude that RICEs act to degrade uridylated 5' products of AGO cleavage to maintain functional RISC. Our study also suggests a possible link between decay of cleaved target mRNAs and miRNA stability in RISC.
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RISC-interacting clearing 3'- 5' exoribonucleases (RICEs) degrade uridylated cleavage fragments to maintain functional RISC in Arabidopsis thaliana.,Zhang Z, Hu F, Sung MW, Shu C, Castillo-Gonzalez C, Koiwa H, Tang G, Dickman M, Li P, Zhang X Elife. 2017 May 2;6. pii: e24466. doi: 10.7554/eLife.24466. PMID:28463111<ref>PMID:28463111</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5v5f" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Li, P]]
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[[Category: Li P]]
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[[Category: Mi-rna]]
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[[Category: Nuclease]]
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[[Category: Transferase]]
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Current revision

Crystal structure of RICE1 (PNT2)

PDB ID 5v5f

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