Linear gramicidin synthase
From Proteopedia
(Difference between revisions)
| (3 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | <StructureSection load='5es5' size='340' side='right' caption=' | + | <StructureSection load='5es5' size='340' side='right' caption='Linear gramicidin synthease subunit A initiation module complex with sulfate (PDB code [[5es5]]).' scene=''> |
== Function == | == Function == | ||
'''Linear gramicidin synthase''' LgrA is a biosynthetic enzyme which synthesizes natural products for therapeutics using a modular synthetic logic where each module adds one amino acid to the growing chain. | '''Linear gramicidin synthase''' LgrA is a biosynthetic enzyme which synthesizes natural products for therapeutics using a modular synthetic logic where each module adds one amino acid to the growing chain. | ||
| - | Thus, LgrA is a non-ribosomal peptide synthetase<ref>PMID:31699907</ref>. | + | Thus, LgrA is a non-ribosomal peptide synthetase<ref>PMID:31699907</ref>. Linear gramicidin is a 15 amino acid long polypeptide which is synthetized by synthases LgrA, LgrB, LgrC, LgrD which comprise 16 modules<ref>PMID:15938641</ref>. |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
== Structural highlights == | == Structural highlights == | ||
| + | LgrA core contains 3 essential domains. Adenylation domain which activates the specific amino acid by adenylation, the thiolation domain which produces a thiol ester and the condensation domain which catalyzes the elongation of the peptide chain<ref>PMID:26762462</ref>. | ||
</StructureSection> | </StructureSection> | ||
| + | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
==3D structures of linear gramicidin synthase subunit A== | ==3D structures of linear gramicidin synthase subunit A== | ||
| Line 28: | Line 26: | ||
== References == | == References == | ||
<references/> | <references/> | ||
| + | [[Category:Topic Page]] | ||
Current revision
| |||||||||||
Updated on 05-October-2020
3D structures of linear gramicidin synthase subunit A
5jnf, 5es6 - BpLgrA 2 domains 2-584 - Brevibacillus parabrevis
6ulz - BpLgrA adenylation domain (mutant) 2-684
5es7 - BpLgrA adenylation domain 2-684 + Val + APC + FON
5es5, 5es8, 5es9 - BpLgrA initiation domain 2-766
6mfw - BpLgrA 4 domains 2-1198
6mfx - BpLgrA 4 domains (mutant) 2-1198
6mfy, 6mg0 - BpLgrA 5 domains 2-1716
6mfz - BpLgrA 2-1802
References
- ↑ Reimer JM, Eivaskhani M, Harb I, Guarne A, Weigt M, Schmeing TM. Structures of a dimodular nonribosomal peptide synthetase reveal conformational flexibility. Science. 2019 Nov 8;366(6466). pii: 366/6466/eaaw4388. doi:, 10.1126/science.aaw4388. PMID:31699907 doi:http://dx.doi.org/10.1126/science.aaw4388
- ↑ Schracke N, Linne U, Mahlert C, Marahiel MA. Synthesis of linear gramicidin requires the cooperation of two independent reductases. Biochemistry. 2005 Jun 14;44(23):8507-13. PMID:15938641 doi:http://dx.doi.org/10.1021/bi050074t
- ↑ Reimer JM, Aloise MN, Harrison PM, Schmeing TM. Synthetic cycle of the initiation module of a formylating nonribosomal peptide synthetase. Nature. 2016 Jan 14;529(7585):239-42. doi: 10.1038/nature16503. PMID:26762462 doi:http://dx.doi.org/10.1038/nature16503
