7cw4
From Proteopedia
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==Acetyl-CoA acetyltransferase from Bacillus cereus ATCC 14579== | ==Acetyl-CoA acetyltransferase from Bacillus cereus ATCC 14579== | ||
| - | <StructureSection load='7cw4' size='340' side='right'caption='[[7cw4]]' scene=''> | + | <StructureSection load='7cw4' size='340' side='right'caption='[[7cw4]], [[Resolution|resolution]] 1.56Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CW4 OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[7cw4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus_ATCC_14579 Bacillus cereus ATCC 14579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CW4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CW4 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.56Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cw4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cw4 OCA], [https://pdbe.org/7cw4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cw4 RCSB], [https://www.ebi.ac.uk/pdbsum/7cw4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cw4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q814S6_BACCR Q814S6_BACCR] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacillus cereus ATCC 14579 is a known polyhydroxybutyrate (PHB)-producing microorganism that possesses genes associated with PHB synthesis such as PhaA, PhaB, and PHA synthases. PhaA (i.e., thiolase) is the first enzyme in the PHA biosynthetic pathway, which catalyze the condensation of two acetyl-CoA molecules to acetoacetyl-CoA. Our study elucidated the crystal structure of PhaA in Bacillus cereus ATCC 14579 (BcTHL) in its apo- and CoA-bound forms. BcTHL adopts a type II biosynthetic thiolase structure by forming a tetramer. The crystal structure of CoA-complexed BcTHL revealed that the substrate binding site of BcTHL is constituted by different residues compared with other known thiolases. Our study also revealed that Arg221, a residue involved in ADP binding, undergoes a positional conformational change upon the binding of the CoA molecule. | ||
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| + | Crystal structure of an acetyl-CoA acetyltransferase from PHB producing bacterium Bacillus cereus ATCC 14579.,Hong J, Park W, Seo H, Kim IK, Kim KJ Biochem Biophys Res Commun. 2020 Sep 21. pii: S0006-291X(20)31789-7. doi:, 10.1016/j.bbrc.2020.09.048. PMID:32972748<ref>PMID:32972748</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7cw4" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Bacillus cereus ATCC 14579]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Hong J]] | [[Category: Hong J]] | ||
[[Category: Kim KJ]] | [[Category: Kim KJ]] | ||
Current revision
Acetyl-CoA acetyltransferase from Bacillus cereus ATCC 14579
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