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| | <StructureSection load='6u1q' size='340' side='right'caption='[[6u1q]], [[Resolution|resolution]] 2.87Å' scene=''> | | <StructureSection load='6u1q' size='340' side='right'caption='[[6u1q]], [[Resolution|resolution]] 2.87Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6u1q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U1Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U1Q FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6u1q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U1Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U1Q FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.87Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">vpsO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u1q OCA], [http://pdbe.org/6u1q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u1q RCSB], [http://www.ebi.ac.uk/pdbsum/6u1q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u1q ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u1q OCA], [https://pdbe.org/6u1q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u1q RCSB], [https://www.ebi.ac.uk/pdbsum/6u1q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u1q ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9KTG5_VIBCH Q9KTG5_VIBCH] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillo virgola del koch trevisan 1884]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Brown, E R]] | + | [[Category: Vibrio cholerae]] |
| - | [[Category: Herbert, K]] | + | [[Category: Brown ER]] |
| - | [[Category: Porcella, M E]] | + | [[Category: Herbert K]] |
| - | [[Category: Rubin, S M]] | + | [[Category: Porcella ME]] |
| - | [[Category: Schwechheimer, C]] | + | [[Category: Rubin SM]] |
| - | [[Category: Tripathi, S M]] | + | [[Category: Schwechheimer C]] |
| - | [[Category: Yildiz, F H]] | + | [[Category: Tripathi SM]] |
| - | [[Category: Auto-phosphorylation]]
| + | [[Category: Yildiz FH]] |
| - | [[Category: Biosynthetic protein]]
| + | |
| - | [[Category: Kinase]]
| + | |
| - | [[Category: Polysaccharide synthesis]]
| + | |
| - | [[Category: Tyrosine kinase]]
| + | |
| Structural highlights
Function
Q9KTG5_VIBCH
Publication Abstract from PubMed
Production of an extracellular matrix is essential for biofilm formation, as this matrix both secures and protects the cells it encases. Mechanisms underlying production and assembly of matrices are poorly understood. Vibrio cholerae, relies heavily on biofilm formation for survival, infectivity, and transmission. Biofilm formation requires Vibrio polysaccharide (VPS), which is produced by vps gene-products, yet the function of these products remains unknown. Here, we demonstrate that the vps gene-products vpsO and vpsU encode respectively for a tyrosine kinase and a cognate tyrosine phosphatase. Collectively, VpsO and VpsU act as a tyrosine phosphoregulatory system to modulate VPS production. We present structures of VpsU and the kinase domain of VpsO, and we report observed autocatalytic tyrosine phosphorylation of the VpsO C-terminal tail. The position and amount of tyrosine phosphorylation in the VpsO C-terminal tail represses VPS production and biofilm formation through a mechanism involving the modulation of VpsO oligomerization. We found that tyrosine phosphorylation enhances stability of VpsO. Regulation of VpsO phosphorylation by the phosphatase VpsU is vital for maintaining native VPS levels. This study provides new insights into the mechanism and regulation of VPS production and establishes general principles of biofilm matrix production and its inhibition.
A tyrosine phosphoregulatory system controls exopolysaccharide biosynthesis and biofilm formation in Vibrio cholerae.,Schwechheimer C, Hebert K, Tripathi S, Singh PK, Floyd KA, Brown ER, Porcella ME, Osorio J, Kiblen JTM, Pagliai FA, Drescher K, Rubin SM, Yildiz FH PLoS Pathog. 2020 Aug 25;16(8):e1008745. doi: 10.1371/journal.ppat.1008745., eCollection 2020 Aug. PMID:32841296[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Schwechheimer C, Hebert K, Tripathi S, Singh PK, Floyd KA, Brown ER, Porcella ME, Osorio J, Kiblen JTM, Pagliai FA, Drescher K, Rubin SM, Yildiz FH. A tyrosine phosphoregulatory system controls exopolysaccharide biosynthesis and biofilm formation in Vibrio cholerae. PLoS Pathog. 2020 Aug 25;16(8):e1008745. doi: 10.1371/journal.ppat.1008745., eCollection 2020 Aug. PMID:32841296 doi:http://dx.doi.org/10.1371/journal.ppat.1008745
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